Davidsen B, Kristensen E
Medical Dept. C, Herlev Hospital, University of Copenhagen, Denmark.
Scand J Gastroenterol. 1987 Sep;22(7):785-90. doi: 10.3109/00365528708991915.
The following immunologic in vitro tests were applied on peripheral blood mononuclear cells (PBMC) from patients with chronic inflammatory bowel disease (IBD): concanavalin A (Con A)-induced suppressor test, Con A-activated lymphoblast transformation test, and spontaneous lymphoblast transformation test. Concomitant phenotypic characterization of subsets of PBMC was performed with monoclonal antibodies. Patients with ulcerative colitis and a control group with rheumatoid arthritis showed significantly reduced activity in the Con A-activated lymphoblast transformation test compared with healthy controls and patients with Crohn's disease. The distribution of PBMC subsets and the results of the other in vitro tests were similar for patients with IBD and healthy controls. Thus the decrease in Con A-activated lymphoblast activity was not due to an increased suppressor function as measured either by functional Con A-induced suppressor test or indirectly by T8 phenotype.
对慢性炎症性肠病（IBD）患者的外周血单个核细胞（PBMC）进行了以下体外免疫学检测：刀豆蛋白A（Con A）诱导的抑制试验、Con A激活的淋巴细胞转化试验和自发淋巴细胞转化试验。使用单克隆抗体对PBMC亚群进行了伴随的表型特征分析。与健康对照和克罗恩病患者相比，溃疡性结肠炎患者和类风湿性关节炎对照组在Con A激活的淋巴细胞转化试验中活性显著降低。IBD患者和健康对照的PBMC亚群分布及其他体外试验结果相似。因此，Con A激活的淋巴细胞活性降低并非由于功能性Con A诱导的抑制试验或通过T8表型间接测量的抑制功能增加所致。
