从植物、动物和人类中分离出的变栖克雷伯菌和准肺炎克雷伯菌的基因组错误分类。
Genome misclassification of Klebsiella variicola and Klebsiella quasipneumoniae isolated from plants, animals and humans.
作者信息
Martínez-Romero Esperanza, Rodríguez-Medina Nadia, Beltrán-Rojel Marilu, Silva-Sánchez Jesús, Barrios-Camacho Humberto, Pérez-Rueda Ernesto, Garza-Ramos Ulises
机构信息
Centro de Ciencias Genómicas, Universidad Nacional Autónoma de México. Cuernavaca, Morelos, México.
Grupo de Resistencia Bacteriana, Centro de Investigación sobre Enfermedades Infecciosas, Instituto Nacional de Salud Pública. Cuernavaca, Morelos, México.
出版信息
Salud Publica Mex. 2018 Jan-Feb;60(1):56-62. doi: 10.21149/8149.
OBJECTIVE
Due to the fact that K. variicola, K. quasipneumoniae and K. pneumoniae are closely related bacterial species, misclassification can occur due to mistakes either in normal biochemical tests or during submission to public databases. The objective of this work was to identify K. variicola and K. quasipneumoniae genomes misclassified in GenBank database.
MATERIALS AND METHODS
Both rpoB phylogenies and average nucleotide identity (ANI) were used to identify a significant number of misclassified Klebsiella spp. genomes.
RESULTS
Here we report an update of K. variicola and K. Quasipneumoniae genomes correctly classified and a list of isolated genomes obtained from humans, plants, animals and insects, described originally as K. pneumoniae or K. variicola, but known now to be misclassified.
CONCLUSIONS
This work contributes to recognize the extensive presence of K. variicola and K. quasipneumoniae isolates in diverse sites and samples.
目的
由于类肺炎克雷伯菌、准肺炎克雷伯菌和肺炎克雷伯菌是密切相关的细菌物种，在常规生化检测或提交至公共数据库时可能会出现错误分类。本研究的目的是识别GenBank数据库中错误分类的类肺炎克雷伯菌和准肺炎克雷伯菌基因组。
材料与方法
利用rpoB系统发育树和平均核苷酸同一性（ANI）来识别大量错误分类的克雷伯菌属基因组。
结果
在此，我们报告了正确分类的类肺炎克雷伯菌和准肺炎克雷伯菌基因组的更新情况，以及从人类、植物、动物和昆虫中分离得到的基因组列表，这些基因组最初被描述为肺炎克雷伯菌或类肺炎克雷伯菌，但现在已知被错误分类。
结论
本研究有助于认识到类肺炎克雷伯菌和准肺炎克雷伯菌分离株在不同场所和样本中的广泛存在。
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