Department of Oral Immunology and Infectious Diseases, University of Louisville School of Dentistry, Louisville, Kentucky, USA.
Bristol Dental School, University of Bristol, Bristol, United Kingdom.
mBio. 2018 Apr 24;9(2):e00202-18. doi: 10.1128/mBio.00202-18.
The pleiomorphic yeast is a significant pathogen in immunocompromised individuals. In the oral cavity, is an inhabitant of polymicrobial communities, and interspecies interactions promote hyphal formation and biofilm formation. colonizes the subgingival area, and the frequency of colonization increases in periodontal disease. In this study, we investigated the interactions between and the periodontal pathogen and were found to coadhere in both the planktonic and sessile phases. Loss of the internalin-family protein InlJ abrogated adhesion of to , and recombinant InlJ protein competitively inhibited interspecies binding. A mutant of deficient in expression of major hyphal protein Als3 showed diminished binding to , and InlJ interacted with Als3 heterologously expressed in Transcriptional profiling by RNA sequencing (RNA-Seq) established that 57 genes were uniquely upregulated in an InlJ-dependent manner in - communities, with overrepresentation of those corresponding to 31 gene ontology terms, including those associated with growth and division. Of potential relevance to the disease process, induced upregulation of components of the type IX secretion apparatus. Collectively, these findings indicate that InlJ-Als3-dependent binding facilitates interdomain community development between and and that has the potential for increased virulence within such communities. Many diseases involve the concerted actions of microorganisms assembled in polymicrobial communities. Inflammatory periodontal diseases are among the most common infections of humans and result in destruction of gum tissue and, ultimately, in loss of teeth. In periodontal disease, pathogenic communities can include the fungus ; however, the contribution of to the synergistic virulence of the community is poorly understood. Here we characterize the interactions between and the keystone bacterial pathogen and show that coadhesion mediated by specific proteins results in major changes in gene expression by , which could serve to increase pathogenic potential. The work provides significant insights into interdomain interactions that can enhance our understanding of diseases involving a multiplicity of microbial pathogens.
多形性酵母是免疫功能低下个体的重要病原体。在口腔中，是多种微生物群落的栖息者，种间相互作用促进了菌丝形成和生物膜形成。定植于龈下区域，且在牙周病中定植频率增加。在这项研究中，我们研究了与牙周病原体之间的相互作用，发现两者在浮游和固着阶段都能共同黏附。缺失内蛋白家族蛋白 InlJ 会破坏对的黏附，重组 InlJ 蛋白能竞争性抑制种间结合。Als3 主要菌丝蛋白表达缺陷的突变体对的结合能力减弱，InlJ 与在中异源表达的 Als3 相互作用。通过 RNA 测序（RNA-Seq）进行的转录谱分析确定，在依赖于 InlJ 的方式中，有 57 个基因被独特地上调，这些基因与 31 个基因本体论术语的过度表达有关，包括与生长和分裂相关的术语。与疾病过程相关的潜在相关性，诱导了型九分泌装置组件的上调。总的来说，这些发现表明，InlJ-Als3 依赖性结合促进了和之间的域间群落发展，并且在这种群落中具有增加毒力的潜力。许多疾病涉及组装在多微生物群落中的微生物的协同作用。炎症性牙周病是人类最常见的感染之一，导致牙龈组织破坏，最终导致牙齿脱落。在牙周病中，致病性群落可能包括真菌；然而，对群落协同毒力的贡献知之甚少。在这里，我们描述了和关键细菌病原体之间的相互作用，并表明特定蛋白介导的共黏附导致了的主要基因表达变化，这可能增加其致病性。这项工作提供了对增强我们对涉及多种微生物病原体的疾病的理解的域间相互作用的重要见解。
