Department of Urology, Peking University Shenzhen Hospital, Shenzhen, Guangdong 518036, P.R. China.
Mol Med Rep. 2018 Jun;17(6):8510-8517. doi: 10.3892/mmr.2018.8899. Epub 2018 Apr 19.
Renal cell carcinoma (RCC) is one of the most common urinary tumors. Previous studies have demonstrated that microRNA (miR)‑181a‑5p has an important role in numerous types of cancer. However, the function of miR‑181a‑5p in RCC remains unknown. In the present study, the expression levels of miR‑181a‑5p in RCC tissues and cell lines were investigated using reverse transcription‑quantitative polymerase chain reaction (RT‑qPCR) analysis. The results of the RT‑qPCR analyses suggested that the expression of miR‑181a‑5p was upregulated in RCC tissues and cells lines compared with adjacent normal renal tissues and normal renal cell lines. Furthermore, the effect of miR‑181a‑5p on cell proliferation, migration, invasion and apoptosis was investigated in the present study. Overexpression of miR‑181a‑5p was revealed to suppress the apoptosis of 786‑O and ACHN cells, in addition to enhancing the proliferation, migration and invasion abilities of 786‑O and ACHN cells in vitro, thus suggesting that miR‑181a‑5p may function as an oncogene in RCC. However, further studies are required to investigate the underlying mechanism of miR‑181a‑5p and its potential role as a biomarker for early detection and prognosis, in addition to as a therapeutic target in RCC.
肾细胞癌（RCC）是最常见的泌尿系统肿瘤之一。先前的研究表明，微小 RNA（miR）-181a-5p 在多种类型的癌症中具有重要作用。然而，miR-181a-5p 在 RCC 中的功能仍不清楚。在本研究中，通过逆转录-定量聚合酶链反应（RT-qPCR）分析研究了 miR-181a-5p 在 RCC 组织和细胞系中的表达水平。RT-qPCR 分析的结果表明，与相邻正常肾组织和正常肾细胞系相比，miR-181a-5p 在 RCC 组织和细胞系中的表达上调。此外，本研究还研究了 miR-181a-5p 对细胞增殖、迁移、侵袭和凋亡的影响。过表达 miR-181a-5p 被发现可抑制 786-O 和 ACHN 细胞的凋亡，同时增强 786-O 和 ACHN 细胞在体外的增殖、迁移和侵袭能力，这表明 miR-181a-5p 可能在 RCC 中作为癌基因发挥作用。然而，需要进一步研究来探讨 miR-181a-5p 的潜在机制及其作为早期检测和预后的生物标志物的作用，以及作为 RCC 治疗靶点的作用。
