Gülbahçe Mutlu E, Arslan E, Öznurlu Y, Özparlak H
a Department of Physiology, Faculty of Medicine , KTO Karatay University , Karatay.
b Department of Biology, Faculty of Science , Selçuk University , Selçuklu , Konya , Turkey.
Biotech Histochem. 2018;93(6):463-470. doi: 10.1080/10520295.2018.1454986. Epub 2018 Apr 25.
Many types of aflatoxin cause problems for both public and animal health. Aflatoxin B (AFB) is the most toxic and commonly encountered fungal toxin that appears in poultry feed and in feeds stored under unsuitable conditions. AFB decreases feed quality, egg production and fertility of hatching eggs. Also, AFB alters the development of embryos by infecting eggs. We investigated using sequence analysis the changes caused by different concentrations of AFB on the promoter sequences of the growth hormone regulated gene-1 (GHRG-1) in chick embryo at 13, 17, 19 and 21 days incubation. DNA isolated from the liver of chick embryos treated with different concentrations of AFB was separated using agarose gel electrophoresis to detect apoptosis, and DNA interaction with AFB was investigated using plasmids to detect changes in electrophoretic mobility and their effects on DNA. Base changes of the promoter sequences of GHRG-1 in 5 ng/egg, 15 ng/egg and 40 ng/egg doses of AFB were increased on day 19 compared to base changes of the same AFB doses on day 13. We also found that AFB at different concentrations changed the mobility of DNA by binding to it, and that high doses of AFB1 destroyed DNA. The DNA interaction study using plasmid demonstrated that AFB at high doses was bound to plasmid DNA, slowed its mobility and inhibited restriction cuts.
多种黄曲霉毒素会对公众健康和动物健康造成问题。黄曲霉毒素B（AFB）是毒性最强且最常见的真菌毒素，出现在家禽饲料以及储存条件不佳的饲料中。AFB会降低饲料质量、产蛋量和孵化蛋的受精率。此外，AFB通过感染鸡蛋改变胚胎发育。我们使用序列分析方法，研究了在孵化第13、17、19和21天，不同浓度的AFB对鸡胚生长激素调节基因-1（GHRG-1）启动子序列的影响。用不同浓度AFB处理的鸡胚肝脏中分离出的DNA，通过琼脂糖凝胶电泳进行分离以检测细胞凋亡，并使用质粒研究DNA与AFB的相互作用，以检测电泳迁移率的变化及其对DNA的影响。与第13天相同AFB剂量的碱基变化相比，第19天5 ng/蛋、15 ng/蛋和40 ng/蛋剂量的AFB处理后，GHRG-1启动子序列的碱基变化增加。我们还发现，不同浓度的AFB通过与DNA结合改变其迁移率，并且高剂量的AFB1会破坏DNA。使用质粒进行的DNA相互作用研究表明，高剂量的AFB与质粒DNA结合，减缓其迁移率并抑制限制性切割。
