Xi Lijun, Qiao Nenghu, Liu Dejian, Li Jing, Zhang Jingjing, Liu Jianguo
State Key Laboratory of Heavy Oil Processing and Centre for Bioengineering and Biotechnology, China University of Petroleum (East China), Qingdao 266580, PR China.
Int J Syst Evol Microbiol. 2018 Jun;68(6):2042-2047. doi: 10.1099/ijsem.0.002794. Epub 2018 Apr 25.
Two bacterial strains were isolated from coal mine water in China. Isolates were facultatively anaerobic, Gram-stain-negative, rod-shaped, motile by means of a single polar flagellum, and they did not produce bacteriochlorophyll α. Cells grew in tryptic soy broth with 0-5.5 % (w/v) NaCl, at 4-55 °C and pH 3.5-10.5. Isolates were positive for catalase, oxidase, urease, Voges-Proskauer test, gelatin hydrolysis and H2S production. Analysis of 16S rRNA gene sequences indicated that the closest relatives of strains Q4.6 and Q2.11 were the type strains Labrenzia suaedae DSM 22153 (97.4 %), Pannonibacter phragmitetus DSM 14782 (96.9 and 97.0 %) and Pannonibacter indicus DSM 23407 (96.8 %). The genomic average nucleotide identity (ANI) value for Q4.6 and Q2.11 was 100 %; however, this value was less than 77.7 % for the type strains P. phragmitetus and P. indicus, and less than 74.0 % for the type strain L. suaedae. The cellular fatty acid profile of strains Q4.6 and Q2.11 consisted primarily of C18 : 1ω7c. The principal quinone of the isolates was Q-10. The polar lipid profile consisted of diphosphatidyl glycerol, phosphatidyl glycerol, phosphatidyl ethanolamine and phosphatidyl choline. On the basis of phylogenetic analysis, genomic ANI analysis, DNA-DNA hybridization results, as well as phenotypic and chemotaxonomic data, strains Q4.6 and Q2.11 are assigned as a novel species within the genus Pannonibacter. The type strain is Pannonibacter carbonis Q4.6 (=CGMCC 1.15703=KCTC 52466).
从中国的煤矿水中分离出两株细菌。分离菌株为兼性厌氧菌，革兰氏染色阴性，杆状，通过单根极鞭毛运动，且不产生细菌叶绿素α。细胞能在含0 - 5.5%（w/v）NaCl的胰蛋白胨大豆肉汤中生长，生长温度为4 - 55°C，pH值为3.5 - 10.5。分离菌株的过氧化氢酶、氧化酶、脲酶、Voges - Proskauer试验、明胶水解和H₂S产生试验均呈阳性。16S rRNA基因序列分析表明，菌株Q4.6和Q2.11最接近的亲缘种是模式菌株Labrenzia suaedae DSM 22153（97.4%）、Pannonibacter phragmitetus DSM 14782（96.9%和97.0%）以及Pannonibacter indicus DSM 23407（96.8%）。菌株Q4.6和Q2.11的基因组平均核苷酸同一性（ANI）值为100%；然而，模式菌株P. phragmitetus和P. indicus的该值小于77.7%，模式菌株L. suaedae的该值小于74.0%。菌株Q4.6和Q2.11的细胞脂肪酸谱主要由C18 : 1ω7c组成。分离菌株的主要醌为Q - 10。极性脂质谱由双磷脂酰甘油、磷脂酰甘油、磷脂酰乙醇胺和磷脂酰胆碱组成。基于系统发育分析、基因组ANI分析、DNA - DNA杂交结果以及表型和化学分类学数据，菌株Q4.6和Q2.11被归为Pannonibacter属内的一个新物种。模式菌株为Pannonibacter carbonis Q4.6（=CGMCC 1.15703 = KCTC 52466）。
