Department of Biotechnology, Institute of Life Sciences, Nalco Square, Bhubaneswar 751 023, India.
Arch Microbiol. 2013 Jan;195(1):1-8. doi: 10.1007/s00203-012-0840-z. Epub 2012 Sep 1.
A novel aerobic bacterium, strain HT23(T), able to grow on 500 mM sodium arsenate was isolated from a hot-spring sediment sample collected from Athamallik, Orissa, India. Cells of this isolate were Gram negative. Heterotrophic growth was observed at pH 6.0-11.0 and 20-45 °C. Optimum growth was observed at 37 °C and pH 7.0-10.0. The major polar lipids are diphosphatidyl glycerol, phosphatidyl glycerol, phosphatidyl ethanolamine, phosphatidyl choline and phosphatidyl monomethyl ethanolamine. The major isoprenoid quinone was Q-10. 16S rRNA gene sequence analysis indicated that the bacterium clustered with the genus Pannonibacter and showed 98.9 % similarity with Pannonibacter phragmitetus C6-19(T) (DSM 14782(T)) and 98 % with the P. phragmitetus group B and P. phragmitetus group E strains. Levels of DNA-DNA relatedness between the strain HT23(T) and P. phragmitetus C6-19(T) (DSM 14782(T)) and other strains of P. phragmitetus group B and group E strains were below 55 %. On the basis of phenotypic and chemotaxonomic characteristics, 16S rRNA gene sequence analysis and DNA-DNA hybridization data, strain HT23(T) is considered to represent a novel species of the genus Pannonibacter, for which the name Pannonibacter indica sp. nov. is proposed. The type strain is HT23(T) (=JCM 16851(T) = DSM 23407(T) = LMG 25769(T)).
一株能够在 500mM 砷酸钠上生长的新型需氧细菌，菌株 HT23(T)，从印度奥里萨邦 Athamallik 的温泉沉积物样品中分离得到。该分离株的细胞呈革兰氏阴性。在 pH6.0-11.0 和 20-45°C 时观察到异养生长。在 37°C 和 pH7.0-10.0 时观察到最佳生长。主要极性脂是双磷脂酰甘油、磷脂酰甘油、磷脂酰乙醇胺、磷脂酰胆碱和磷脂酰单甲基乙醇胺。主要的异戊烯醌是 Q-10。16S rRNA 基因序列分析表明，该细菌与 Pannonibacter 属聚类，并与 Pannonibacter phragmitetus C6-19(T) (DSM 14782(T)) 具有 98.9%的相似性，与 P. phragmitetus 组 B 和 P. phragmitetus 组 E 菌株具有 98%的相似性。菌株 HT23(T)与 P. phragmitetus C6-19(T) (DSM 14782(T)) 和 P. phragmitetus 组 B 和组 E 菌株的其他菌株之间的 DNA-DNA 同源性水平低于 55%。基于表型和化学分类特征、16S rRNA 基因序列分析和 DNA-DNA 杂交数据，菌株 HT23(T)被认为代表了 Pannonibacter 属的一个新种，建议将其命名为 Pannonibacter indica sp. nov.。模式菌株为 HT23(T) (=JCM 16851(T) =DSM 23407(T) =LMG 25769(T))。
