College of Forestry and Landscape Architecture, Anhui Agricultural University, Hefei, Anhui, China.
Key laboratory of High Magnetic Field and Ion Beam Physical Biology, Hefei Institutes of Physical Science, Chinese Academy of Sciences, Hefei, Anhui, PR China.
PLoS One. 2018 Apr 25;13(4):e0195913. doi: 10.1371/journal.pone.0195913. eCollection 2018.
Pecan is an economically important nut crop tree due to its unique texture and flavor properties. The pecan seed is rich of unsaturated fatty acid and protein. However, little is known about the molecular mechanisms of the biosynthesis of fatty acids in the developing seeds. In this study, transcriptome sequencing of the developing seeds was performed using Illumina sequencing technology. Pecan seed embryos at different developmental stages were collected and sequenced. The transcriptomes of pecan seeds at two key developing stages (PA, the initial stage and PS, the fast oil accumulation stage) were also compared. A total of 82,155 unigenes, with an average length of 1,198 bp from seven independent libraries were generated. After functional annotations, we detected approximately 55,854 CDS, among which, 2,807 were Transcription Factor (TF) coding unigenes. Further, there were 13,325 unigenes that showed a 2-fold or greater expression difference between the two groups of libraries (two developmental stages). After transcriptome analysis, we identified abundant unigenes that could be involved in fatty acid biosynthesis, degradation and some other aspects of seed development in pecan. This study presents a comprehensive dataset of transcriptomic changes during the seed development of pecan. It provides insights in understanding the molecular mechanisms responsible for fatty acid biosynthesis in the seed development. The identification of functional genes will also be useful for the molecular breeding work of pecan.
山核桃是一种具有独特质地和风味的经济上重要的坚果作物。山核桃种子富含不饱和脂肪酸和蛋白质。然而，对于发育中的种子中脂肪酸生物合成的分子机制知之甚少。在这项研究中，使用 Illumina 测序技术对发育中的种子进行了转录组测序。收集了不同发育阶段的山核桃种子胚胎并进行了测序。还比较了山核桃种子在两个关键发育阶段（PA，初始阶段和 PS，快速油积累阶段）的转录组。从七个独立文库中总共生成了 82155 个平均长度为 1198bp 的 unigenes。经过功能注释，我们检测到大约 55854 个 CDS，其中 2807 个是转录因子（TF）编码的 unigenes。此外，有 13325 个 unigenes在两组文库（两个发育阶段）之间的表达差异达到 2 倍或更高。通过转录组分析，我们鉴定了丰富的 unigenes，它们可能参与山核桃种子发育过程中的脂肪酸生物合成、降解和种子发育的其他方面。本研究提供了山核桃种子发育过程中转录组变化的综合数据集。它为理解种子发育中脂肪酸生物合成的分子机制提供了新的见解。功能基因的鉴定也将对山核桃的分子育种工作有用。
