Matia-García Inés, Salgado-Goytia Lorenzo, Ramos-Arellano Luz Elena, Muñoz-Valle José Francisco, Armenta-Solís Adakatia, Garibay-Cerdenares Olga Lilia, Ramírez Mónica, Parra-Rojas Isela
Facultad de Ciencias Químico Biológicas, Universidad Autónoma de Guerrero, Chilpancingo, Guerrero, México.
Departamento de Biología Molecular y Genómica, Centro Universitario de Ciencias de la Salud, Instituto de Investigación en Ciencias Biomédicas, Universidad de Guadalajara, Jalisco, México.
Arch Endocrinol Metab. 2018 Feb;62(1):79-86. doi: 10.20945/2359-3997000000012.
Objective Monocyte chemoattractant protein 1 (MCP-1) has been suggested to be involved in the pathophysiology of insulin resistance (IR); therefore, variants in the MCP-1 gene may contribute to the development of this disease. The aim of this study was to analyze the relationship of the -2518 A>G MCP-1 (rs1024611) gene polymorphism with insulin resistance in Mexican children. Subjects and methods A cross-sectional study was performed in 174 children, including 117 children without insulin resistance and 57 children with IR, with an age range of 6-11 years. Levels for serum insulin and high-sensitivity C-reactive protein were determined. The -2518 A>G MCP-1 polymorphism was identified by the polymerase chain reaction-restriction fragment length polymorphism method. Insulin resistance was defined as a HOMA-IR in the upper 75th percentile, which was ≥ 2.4 for all children. Results Genotype frequencies of the rs1024611 polymorphism for the insulin-sensitive group were 17% AA, 48% AG and 35% GG, and the frequency of G allele was 59%, whereas frequencies for the insulin-resistant group were 12% AA, 37% AG and 51% GG, and the frequency of G allele was 69%. The genotype and allele frequencies between groups did not show significant differences. However, the GG genotype was the most frequent in children with IR. The GG genotype was associated with insulin resistance (OR = 2.2, P = 0.03) in a genetic model. Conclusion The -2518 A>G MCP-1 gene polymorphism may be related to the development of insulin resistance in Mexican children.
目的 单核细胞趋化蛋白1（MCP-1）被认为参与了胰岛素抵抗（IR）的病理生理过程；因此，MCP-1基因的变异可能促成该疾病的发生。本研究的目的是分析-2518 A>G MCP-1（rs1024611）基因多态性与墨西哥儿童胰岛素抵抗之间的关系。
对象与方法 对174名儿童进行了一项横断面研究，其中包括117名无胰岛素抵抗的儿童和57名患有IR的儿童，年龄范围为6至11岁。测定了血清胰岛素和高敏C反应蛋白水平。采用聚合酶链反应-限制性片段长度多态性方法鉴定-2518 A>G MCP-1多态性。胰岛素抵抗定义为稳态模型评估的胰岛素抵抗指数（HOMA-IR）处于第75百分位数以上，所有儿童的该指数≥2.4。
结果 胰岛素敏感组rs1024611多态性的基因型频率为AA占17%、AG占48%、GG占35%，G等位基因频率为59%；而胰岛素抵抗组的频率分别为AA占12%、AG占37%、GG占51%，G等位基因频率为69%。两组间的基因型和等位基因频率无显著差异。然而，GG基因型在患有IR的儿童中最为常见。在遗传模型中，GG基因型与胰岛素抵抗相关（比值比=2.2，P=0.03）。
结论 -2518 A>G MCP-1基因多态性可能与墨西哥儿童胰岛素抵抗的发生有关。
