Wang L, Yang L, Gao L, Gao T W, Li W, Liu Y F
Department of Dermatology, Xijing Hospital, Fourth Military Medical University, Xian, China.
Int J Immunogenet. 2008 Feb;35(1):45-9. doi: 10.1111/j.1744-313X.2007.00734.x. Epub 2007 Dec 18.
Psoriasis is one of the most common inflammatory diseases of skin. MCP-1 is an important CC-type chemokine responsible for monocytes and T lymphocytes recruitment in inflammatory conditions. A single nucleotide polymorphism of the MCP-1 gene in the gene regulatory region was found to be related to the expression of MCP-1, and the associations of this polymorphism with many inflammatory diseases were conformed. However, the significance of this polymorphism in psoriasis remains unclear. Therefore, we examined this polymorphism in 507 patients with plaque-type psoriasis and 530 healthy controls using the polymerase chain reaction-restriction fragment length polymorphism method. We also tested the serum MCP-1 in 320 patients and 160 controls and compared the serum MCP-1 level in patients of different genotypes. Our results showed that the frequency distribution of the AA, AG and GG genotypes between the patients and the controls was statistically different (P = 0.031); significantly increased risk for psoriasis was associated with the AG, GG and AG + GG genotype. The frequency distribution of the AA, AG and GG genotypes was also different between female psoriasis patients and controls (P = 0.025), between type I psoriasis patients and controls (P = 0.025), between psoriasis patients without positive familial history and controls (P = 0.048), and between patients with psoriasis area and severity index of > or = 10 and controls (P = 0.041). MCP-1 serum level was significantly higher in patients than controls (P < 0.0001). There were significant differences of serum MCP-1 level between patients of the GG genotype and the AA genotype (P = 0.028), between patients of the AG genotype and the AA genotype (P = 0.049), and between patients of the AG + GG genotype and the AA genotype (P = 0.027). These results showed the -2518 MCP-1 polymorphism is related to the susceptibility of plaque type psoriasis. Individuals containing the GG or AG genotype were at higher risk of psoriasis than subjects with the AA genotype.
银屑病是最常见的皮肤炎症性疾病之一。MCP-1是一种重要的CC型趋化因子，在炎症状态下负责单核细胞和T淋巴细胞的募集。发现MCP-1基因在基因调控区域的单核苷酸多态性与MCP-1的表达相关，并且这种多态性与许多炎症性疾病的关联也得到了证实。然而，这种多态性在银屑病中的意义仍不清楚。因此，我们采用聚合酶链反应-限制性片段长度多态性方法，对507例斑块型银屑病患者和530例健康对照者进行了该多态性检测。我们还检测了320例患者和160例对照者的血清MCP-1，并比较了不同基因型患者的血清MCP-1水平。我们的结果显示，患者和对照者之间AA、AG和GG基因型的频率分布在统计学上存在差异（P = 0.031）；AG、GG和AG + GG基因型与银屑病风险显著增加相关。女性银屑病患者和对照者之间、I型银屑病患者和对照者之间、无阳性家族史的银屑病患者和对照者之间，以及银屑病面积和严重程度指数≥10的患者和对照者之间，AA、AG和GG基因型的频率分布也存在差异（P = 0.025、P = 0.025、P = 0.048、P = 0.041）。患者的MCP-1血清水平显著高于对照者（P < 0.0001）。GG基因型患者与AA基因型患者之间、AG基因型患者与AA基因型患者之间以及AG + GG基因型患者与AA基因型患者之间的血清MCP-1水平存在显著差异（P = 0.028、P = 0.049、P = 0.027）。这些结果表明，MCP-1基因-2518位点的多态性与斑块型银屑病的易感性相关。携带GG或AG基因型的个体患银屑病的风险高于携带AA基因型的个体。
