Kelleher Philip, Murphy James, Mahony Jennifer, van Sinderen Douwe
School of Microbiology, University College Cork, Cork, Ireland.
Alimentary Pharmabiotic Centre, University College Cork, Cork, Ireland.
Methods Mol Biol. 2018;1681:127-137. doi: 10.1007/978-1-4939-7343-9_10.
Whole phage genomes can be sequenced readily using one or a combination of next generation sequencing (NGS) technologies. One of the most recently developed NGS platforms, the so-called Single-Molecule Real-Time (SMRT) sequencing approach provided by the PacBio RS platform, is particularly useful in providing complete (i.e., un-gapped) genome sequences, but differs from other technologies in that the platform also allows for downstream analysis to identify nucleotides that have been modified by DNA methylation. Here, we describe the methodological approach for the detection of genomic methylation motifs by means of SMRT sequencing.
完整的噬菌体基因组可以使用一种或多种下一代测序（NGS）技术轻松测序。PacBio RS平台提供的最新开发的NGS平台之一，即所谓的单分子实时（SMRT）测序方法，在提供完整（即无间隙）基因组序列方面特别有用，但与其他技术不同的是，该平台还允许进行下游分析，以识别被DNA甲基化修饰的核苷酸。在这里，我们描述了通过SMRT测序检测基因组甲基化基序的方法。
