Farcic Thiago Saikali, Baldan Cristiano Schiavinato, Machado Aline Fernanda Perez, Caffaro Leonardo Affonso Massabki, Masson Igor Fagioli Bordello, Casarotto Raquel Aparecida
Department of Physical Therapy, Faculdade de Medicina, Universidade de São Paulo (FMUSP), São Paulo, Brazil.
Department of Physical Therapy, Universidade Paulista (UNIP), São Paulo, Brazil.
Adv Wound Care (New Rochelle). 2018 Apr 1;7(4):114-120. doi: 10.1089/wound.2017.0748.
The aim of this study was to evaluate the organization of collagen fibers in the healing process of rat Achilles tendon rupture using different times of ultrasound therapy (TUS). Forty Wistar rats were selected. Among these, 32 were submitted to total and divided into 5 groups: control group (CG, = 8), without tenotomy or any treatment; tenotomy group ( = 8), with tenotomy and without treatment; TUS groups-TUS3 ( = 8), TUS4 ( = 8), and TUS5 ( = 8)-submitted to tenotomy and treated with TUS for 3, 4, and 5 min per effective radiating area (ERA), respectively. The animals were sacrificed on the 12th postoperative day. The tendons were surgically removed for analysis of the collagen fiber organization using the birefringence technique (OR, optical retardation). The collagen fibers exhibited better aggregation and organization in the UST3, TUS4, and TUS5 groups compared with CG ( < 0.05). The TUS5 group had rates in intergroup comparison. The dose response of therapeutic TUS is influenced by many variables. The scientific evidence to support the dosimetry is insufficient. The application time is an important variable to be considered in TUS. In this study, the longer the application time, the better for organization and aggregation of collagen fibers in the rat tendon. TUS applied for 5 min per ERA presented higher dose response to the organization of collagen fibers in the healing process of rat Achilles tendon rupture.
本研究的目的是使用不同时长的超声治疗（TUS）来评估大鼠跟腱断裂愈合过程中胶原纤维的组织情况。选取了40只Wistar大鼠。其中，32只接受了完全切断手术并分为5组：对照组（CG，n = 8），未进行跟腱切断术或任何治疗；跟腱切断组（n = 8），进行了跟腱切断术但未治疗；TUS组——TUS3组（n = 8）、TUS4组（n = 8）和TUS5组（n = 8）——进行了跟腱切断术，并分别以每个有效辐射面积（ERA）3、4和5分钟的时长接受TUS治疗。术后第12天处死动物。手术取出肌腱，使用双折射技术（OR，光学延迟）分析胶原纤维的组织情况。与CG组相比，UST3组、TUS4组和TUS5组的胶原纤维表现出更好的聚集和组织情况（P < 0.05）。TUS5组在组间比较中具有[此处原文缺失具体数据内容]率。治疗性TUS的剂量反应受许多变量影响。支持剂量测定法的科学证据不足。在TUS中，应用时间是一个需要考虑的重要变量。在本研究中，应用时间越长，对大鼠肌腱中胶原纤维的组织和聚集就越好。每个ERA应用5分钟的TUS在大鼠跟腱断裂愈合过程中对胶原纤维组织呈现出更高的剂量反应。
