Moura Júnior Manoel de Jesus, Arisawa Emilia Ângela Loschiavo, Martin Airton Abrahão, de Carvalho Janderson Pereira, da Silva José Mário Nunes, Silva José Figueiredo, Silveira Landulfo
Biomedical Engineering Institute, Universidade Camilo Castelo Branco-UNICASTELO, Parque Tecnológico de São José dos Campos, Estr. Dr. Altino Bondesan, 500, São José dos Campos, São Paulo, Brazil, 12247-016,
Lasers Med Sci. 2014 Jan;29(1):301-11. doi: 10.1007/s10103-013-1327-0. Epub 2013 May 10.
This work evaluated the anti-inflammatory response of low-power light-emitting diode (LED) and ultrasound (US) therapies and the quality and rapidness of tendon repair in an experimental model of tendinitis, employing histomorphometry and Raman spectroscopy. Tendinitis was induced by collagenase into the right tendon of 35 male Wistar rats with an average weight of 230 g. The animals were randomly separated into seven groups of five animals each: tendinitis without treatment-control (TD7 and TD14, where 1 and 2 indicated sacrifice on the 7th and 14th day, respectively), tendinitis submitted to US therapy (US7 and US14) and tendinitis submitted to LED therapy (LED7 and LED14). Contralateral tendons of the TD group at the 14th day were used as the healthy group (H). US treatment was applied in pulsed mode at 10 %, 1 MHz frequency, 0.5 W/cm(2), 120 s. LED therapy parameters were 4 J/cm(2), 120 s, daily dose at the same time and same point. Sacrifice was performed on the 7th or 14th day. Histomorphometric analysis showed lower number of fibroblasts on the 14th day of therapy for the US-treated group, compared to the TD and LED, indicating lower tissue inflammation. Raman showed that the LED group had an increase in the amount of collagen I and III from the 7th to the 14th day, which would indicate more organized fibers and a better quality of the healing, and US showed lower collagen I synthesis in the 14th day compared to H, indicating a lower tissue reorganization.
本研究在肌腱炎实验模型中，采用组织形态计量学和拉曼光谱法，评估了低功率发光二极管（LED）和超声（US）疗法的抗炎反应以及肌腱修复的质量和速度。通过向35只平均体重为230 g的雄性Wistar大鼠的右侧肌腱注射胶原酶诱导肌腱炎。将动物随机分为7组，每组5只：未治疗的肌腱炎对照组（TD7和TD14，其中1和2分别表示在第7天和第14天处死）、接受US疗法的肌腱炎组（US7和US14）以及接受LED疗法的肌腱炎组（LED7和LED14）。将TD组第14天的对侧肌腱用作健康组（H）。US治疗以脉冲模式进行，频率为1 MHz，强度为0.5 W/cm²，持续120 s，占空比为10%。LED疗法参数为4 J/cm²，持续120 s，每天在同一时间、同一点给药。在第7天或第14天处死动物。组织形态计量学分析显示，与TD组和LED组相比，US治疗组在治疗第14天时成纤维细胞数量较少，表明组织炎症较低。拉曼光谱显示，LED组从第7天到第14天I型和III型胶原蛋白含量增加，这表明纤维更有序，愈合质量更好；而US组在第14天时I型胶原蛋白合成低于H组，表明组织重组程度较低。
