Fortin Olivier, Aguilar-Uscanga Blanca R, Vu Khanh D, Salmieri Stephane, Lacroix Monique
a INRS-Institut Armand-Frappier, Research Laboratories in Sciences Applied to Food , Laval , Quebec , Canada.
b Laboratorio de Microbiología Industrial, Centro Universitario de Ciencias Exactas e Ingenierías, Universidad de Guadalajara (UdG) , Jalisco , Mexico.
Nutr Cancer. 2018 May-Jun;70(4):632-642. doi: 10.1080/01635581.2018.1460672. Epub 2018 Apr 26.
The effect of Saccharomyces boulardii cell wall extracts on colon cancer prevention in rats treated with 1,2-dimethylhydrazine was investigated. A crude insoluble glucan (0.5 and 1.0 mg/kg/day) and a crude mannoprotein extract (0.3 and 3.0 mg/kg/day) were administered in rats by gavage for 12 weeks along with a high fat low fiber diet whereupon rats were sacrificed and aberrant crypt foci (ACF) were counted in the colon. Moreover, NAD(P)H: quinone reductase (QR) and harmful fecal enzymes (β-glucosidase and β-glucuronidase) were quantified in the liver and in the caecum, respectively. Results showed a reduction in ACF counts, a decreased β-glucuronidase activity and an increased QR activity when rats were treated only with insoluble glucan. While these enzymatic modulations may be constituted one of the mechanisms that is responsible for the reduction of ACF counts observed, the reduction of ACF counts caused by insoluble glucan should be addressed, at least, as a biomarker of their cancer-prevention properties. To our knowledge, this is the first study demonstrated that crude cell wall extract obtained from S. boulardii could have a potential role in colon cancer prevention in vivo by revealing the potential implication of QR and β-glucuronidase modulation.
研究了布拉酵母菌细胞壁提取物对用1,2 - 二甲基肼处理的大鼠结肠癌预防的作用。将粗制不溶性葡聚糖（0.5和1.0毫克/千克/天）和粗制甘露糖蛋白提取物（0.3和3.0毫克/千克/天）通过灌胃给予大鼠12周，同时给予高脂肪低纤维饮食，然后处死大鼠并计数结肠中的异常隐窝病灶（ACF）。此外，分别在肝脏和盲肠中对NAD(P)H：醌还原酶（QR）和有害粪便酶（β - 葡萄糖苷酶和β - 葡萄糖醛酸酶）进行定量。结果显示，仅用不溶性葡聚糖处理大鼠时，ACF计数减少，β - 葡萄糖醛酸酶活性降低，QR活性增加。虽然这些酶调节可能是导致观察到的ACF计数减少的机制之一，但由不溶性葡聚糖引起的ACF计数减少至少应作为其癌症预防特性的生物标志物。据我们所知，这是第一项研究表明，从布拉酵母菌获得的粗制细胞壁提取物通过揭示QR和β - 葡萄糖醛酸酶调节的潜在影响，可能在体内结肠癌预防中发挥潜在作用。
