Pakbin Babak, Pishkhan Dibazar Shaghayegh, Allahyari Samaneh, Javadi Maryam, Farasat Alireza, Darzi Sina
Department of Food Hygiene and Quality of Control Faculty of Veterinary Medicine University of Tehran Tehran Iran.
Department of Immunology Faculty of Medical Science Tarbiat Modares University Tehran Iran.
Food Sci Nutr. 2020 Nov 29;9(2):692-700. doi: 10.1002/fsn3.2032. eCollection 2021 Feb.
Natural anticancer drug and compounds with other great benefits are of interest recently due to lower side effects than chemotherapy for cancer treatment and prevention. Different natural and synthetic drugs have been suggested to be used for treatment of gastric cancers, the second deadly cancer worldwide. The aim of this study was to investigate anticancer activity of SBS including inducing apoptosis and inhibition of survivin gene expression in gastric cancer cells. We evaluated cell viability, inducing apoptosis and change in survivin gene expression of EPG85-257P (EPG) and EPG85-257RDB (resistant to Daunorubicin, RDB) cell lines under exposure of SBS after 24, 48, and 72 hr. We found that SBS decreased cell viability, induced apoptosis, and reduced survivin gene expression in treated EPG and RDB cells (with the significant IC values of 387 and 575 µg/ml after 72 and 48 hr for EPG and RDB cells respectively). However, we observed SBS was more efficient to induce apoptosis in EPG than RDB cells. We strongly suggest SBS be considered as a prospective anticancer agent or in formulation of complementary medication to treat and prevent gastric cancers.
由于与化疗相比副作用更低，可用于癌症治疗和预防，天然抗癌药物及具有其他重大益处的化合物近来备受关注。不同的天然和合成药物已被建议用于治疗胃癌，胃癌是全球第二大致命癌症。本研究的目的是调查SBS在胃癌细胞中诱导凋亡和抑制生存素基因表达的抗癌活性。我们评估了在暴露于SBS 24、48和72小时后，EPG85-257P（EPG）和EPG85-257RDB（对柔红霉素耐药，RDB）细胞系的细胞活力、诱导凋亡情况以及生存素基因表达的变化。我们发现SBS降低了处理后的EPG和RDB细胞的细胞活力，诱导了凋亡，并降低了生存素基因表达（EPG和RDB细胞在72小时和48小时后的显著IC值分别为387和575µg/ml）。然而，我们观察到SBS在EPG细胞中比在RDB细胞中诱导凋亡更有效。我们强烈建议将SBS视为一种有前景的抗癌药物，或用于配制辅助药物以治疗和预防胃癌。
