铀酰盐作为用于DNA切割和蛋白质-DNA相互作用探测的光化学试剂。
Uranyl salts as photochemical agents for cleavage of DNA and probing of protein-DNA contacts.
作者信息
Nielsen P E, Jeppesen C, Buchardt O
机构信息
Department of Biochemistry B, Panum Institute, University of Copenhagen, Denmark.
出版信息
FEBS Lett. 1988 Aug 1;235(1-2):122-4. doi: 10.1016/0014-5793(88)81245-6.
PMID:2969827
Abstract
Single-strand DNA nicks are induced by uranyl nitrate or uranyl acetate in combination with long-wavelength (lambda approximately 420 nm) ultraviolet irradiation. The nicks occur randomly with respect to the DNA sequence. Using the lambda-repressor/ORI operator DNA system it is shown that uranyl salts can be used to photofootprint protein contacts with the DNA backbone.
摘要
单链DNA切口可由硝酸铀酰或醋酸铀酰与长波长（约420纳米）紫外线照射联合诱导产生。这些切口相对于DNA序列是随机出现的。利用λ阻遏物/ORI操纵子DNA系统表明，铀盐可用于光足迹法检测蛋白质与DNA骨架的接触。
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