Fujinoki Masakatsu
Department of Physiology, School of Medicine Dokkyo Medical University 321-0293 Mibu Tochigi Japan.
Reprod Med Biol. 2014 Jan 5;13(3):143-152. doi: 10.1007/s12522-013-0175-8. eCollection 2014 Jul.
Hyperactivation of hamster sperm is dose-dependently enhanced by progesterone (P) and 17β-estradiol (E). In the first part of the present study, enhancement of hyperactivation in response to the concentrations of P and E was examined in detail and in the second part, it was examined whether enhancement of hyperactivation by P and E was disrupted by diethylstilbestrol (DES).
Hamster spermatozoa were hyperactivated by incubation in modified Tyrode's albumin lactate pyruvate medium with P, E and/or DES. After spermatozoa were recorded using a video-microscope, observations were quantified by manually counting the numbers of total, motile and hyperactivated spermatozoa.
Hyperactivation was enhanced in response to the concentrations of P and E. When spermatozoa were exposed to DES with E, moreover, DES significantly and strongly suppressed P-enhanced hyperactivation by accelerating the effect of E, but DES itself only weakly suppressed P-enhanced hyperactivation.
Enhancement of hyperactivation was regulated by the concentrations of P and E, suggesting that in vivo hamster spermatozoa are hyperactivated through "monitoring" these concentrations in the oviduct. DES in combination with E suppressed P-enhanced hyperactivation, suggesting that DES significantly disrupts hyperactivation by acting as an accelerator of the effect of E.
孕酮（P）和17β-雌二醇（E）可剂量依赖性地增强仓鼠精子的超活化。在本研究的第一部分，详细研究了P和E浓度对超活化增强的影响，在第二部分中，研究了己烯雌酚（DES）是否会破坏P和E对超活化的增强作用。
将仓鼠精子在含有P、E和/或DES的改良Tyrode白蛋白乳酸丙酮酸培养基中孵育以使其超活化。使用视频显微镜记录精子后，通过手动计数总精子、活动精子和超活化精子的数量对观察结果进行量化。
超活化随P和E的浓度增加而增强。此外，当精子与E一起暴露于DES时，DES通过加速E的作用显著且强烈地抑制了P增强的超活化，但DES本身仅微弱地抑制P增强的超活化。
超活化的增强受P和E浓度的调节，这表明在体内仓鼠精子通过“监测”输卵管中的这些浓度而超活化。DES与E联合使用可抑制P增强的超活化，这表明DES通过作为E作用的促进剂而显著破坏超活化。
