Kyono Koichi, Fukunaga Noritaka, Chiba Setsuyo, Nakajo Yukiko, Fuchinoue Kohei, Yagi Akiko, Araki Yasuhisa
Kyono Reproduction Research Center and Ladies Clinic Kyono, Furukawa, Miyagi and.
The Institute for Advanced Reproductive Medical Technology (ARMT), Fujimi, Setagun, Gunma, Japan.
Reprod Med Biol. 2003 Sep 26;2(3):133-137. doi: 10.1046/j.1445-5781.2003.00031.x. eCollection 2003 Sep.
To evaluate and confirm the merit of two consecutive transfer attempts of early embryos and blastocysts. A total of 685 patients underwent routine fertilization (IVF) cycles. The study population consisted of three groups: (i) Group 1, early stage embryos were transferred (460 patients; 567 cycles); (ii) group 2, blastocysts were transferred (88 patients; 105 cycles); and (iii) group 3, a two-step (consecutive) transfer was performed on 137 women (141 cycles). Specifically, a standard embryo transfer was performed on day 3, together with a two-step (consecutive) transfer of blastocysts. After the early embryo transfer, an extended culture of supernumerary embryos was conducted, followed by a second transfer of blastocyst(s). No significant differences were found in the three groups with regards to either pregnancy or implantation rates for groups 1, 2, or 3; pregnancy: 34.6, 29.9, and 33.6%, respectively; implantation: 18.6, 15.9, and 15.1%, respectively. The miscarriage rate for each group was also not significant; 20.4, 30.8 and 28.6% for groups 1, 2 and 3, respectively. The multiple pregnancy rate of the three groups was 30.4, 30.8 and 35.7%, respectively, and these were not found to be significant results. No significant difference was found between the three groups with regards to the pregnancy, implantation, multiple pregnancy and miscarriage rates. The multiple pregnancy rate was highest in the two-step (consecutive) transfer group. This difference was not regarded to be highly significant. (Reprod Med Biol 2003; : 133-137).
评估并确认早期胚胎和囊胚连续两次移植尝试的价值。共有685例患者接受了常规体外受精（IVF）周期治疗。研究人群分为三组：（i）第1组，移植早期胚胎（460例患者；567个周期）；（ii）第2组，移植囊胚（88例患者；105个周期）；（iii）第3组，对137名女性进行两步（连续）移植（141个周期）。具体而言，在第3天进行标准胚胎移植，同时进行囊胚的两步（连续）移植。早期胚胎移植后，对多余胚胎进行延长培养，随后进行第二次囊胚移植。第1、2或3组在妊娠率或着床率方面，三组之间未发现显著差异；妊娠率分别为34.6%、29.9%和33.6%；着床率分别为18.6%、15.9%和15.1%。每组的流产率也无显著差异；第1、2和3组分别为20.4%、30.8%和28.6%。三组的多胎妊娠率分别为30.4%、30.8%和35.7%，这些结果无显著差异。三组在妊娠、着床、多胎妊娠和流产率方面未发现显著差异。两步（连续）移植组的多胎妊娠率最高。这种差异被认为不具有高度显著性。（《生殖医学与生物学》2003年；：133 - 137）
