Fukunaga N, Haigo K, Kyono K, Araki Y
Ladies Clinic Kyono, Furukawa, Miyagi, Japan.
J Assist Reprod Genet. 2001 Dec;18(12):634-7. doi: 10.1023/a:1013107132110.
To compare fertilization and pregnancy rates of fresh and frozen-thawed testicular sperm injections (TESE-ICSI).
Sperm collected from the testes of 28 azoospermic patients by an open testicular biopsy technique was used for initial ICSI or cryopreserved.
Fresh-sperm ICSI treatment (28 cycles) resulted in a 58.1% fertilization rate and a 32.1% clinical pregnancy rate per embryo transfer, while frozen-thawed sperm (24 subsequent cycles) had rates of 54.5 and 29.2%, respectively. The PR was lower using frozen-thawed sperm from nonobstructive azoospermia patients (9.1%) than from obstructive azoospermia patients (46.2%). PR declined to 0% upon the fourth ICSI attempt.
Fertilization, embryo cleavage, and pregnancy rates were unaffected by fresh or frozen-thawed sperm use. A 57.1% cumulative clinical PR was achieved using the latter. The PR was significantly lower using frozen-thawed sperm from nonobstructive azoospermia patients than from obstructive azoospermia patients.
比较新鲜和冻融睾丸精子注射（TESE-ICSI）的受精率和妊娠率。
通过开放式睾丸活检技术从28例无精子症患者的睾丸中采集精子，用于初始ICSI或冷冻保存。
新鲜精子ICSI治疗（28个周期）每个胚胎移植的受精率为58.1%，临床妊娠率为32.1%，而冻融精子（随后24个周期）的受精率和临床妊娠率分别为54.5%和29.2%。非梗阻性无精子症患者的冻融精子妊娠率（9.1%）低于梗阻性无精子症患者（46.2%）。第四次ICSI尝试时妊娠率降至0%。
新鲜或冻融精子的使用不影响受精、胚胎分裂和妊娠率。使用冻融精子可实现57.1%的累积临床妊娠率。非梗阻性无精子症患者的冻融精子妊娠率显著低于梗阻性无精子症患者。
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