De Placido G, Alviggi C, Clarizia R, Mollo A, Alviggi E, Strina I, Fiore E, Wilding M, Pagano T, Matarese G
Department of Obstetrics Gynecology and Reproductive Medicine, University of Naples Federico II, 80128 Naples, Italy.
J Endocrinol Invest. 2006 Sep;29(8):719-26. doi: 10.1007/BF03344182.
Granulosa-cells are able to produce and store leptin, suggesting that this hormone is locally involved in the regulation of follicular growth. In this study, the role of follicular fluid (FF) leptin concentration in predicting oocyte fertilization and embryo quality was evaluated in 35 normogonadotrophic women undergoing controlled ovarian stimulation (COS) for assisted reproductive techniques.
Leptin concentration was measured in 47 consecutively collected FF in which a mature oocyte had been found during the ovum pick-up. Embryos deriving from fertilized oocytes were submitted to quality scoring systems.
Mean leptin concentration was significantly higher in FF whose oocytes showed 2 pronuclei (no. 25) when compared with those with no evidence of fertilization (no. 22) at the 16-18 h check (26.0+/-6.1 vs 15.3+/-10.6 ng/ml, respectively, p<0.01). Follicular mean diameters were similar in the two groups (21.4+/-3.4 and 21.0+/-5.1 mm, respectively). Logistic regression analysis identified FF leptin levels as the best predictive parameter for oocyte fertilization (p<0.001). When receiving operating characteristics curve was employed, a FF leptin concentration of 20.25 ng/ml was the most reliable cut-off in predicting fertilization of oocytes. FF with leptin concentrations higher than this value (no. 27) had an oocyte fertilization rate of 85.7%. In contrast, FF levels < or =20.25 ng/ml (no. 20) were associated with a rate of 16.7% (p<0.05). No correlation emerged between FF leptin and the score attributed to 15 valuable embryos at the zygote stage (r=-0.01) and at 48 h after insemination (r=0.1).
FF leptin levels are a better predictor of oocyte fertilization success rates than follicular diameter. These results underline the relevance of FF variables in developing methods for oocyte selection.
颗粒细胞能够产生并储存瘦素，这表明该激素局部参与卵泡生长的调节。在本研究中，对35名接受辅助生殖技术控制性卵巢刺激（COS）的正常促性腺激素分泌女性，评估了卵泡液（FF）瘦素浓度在预测卵母细胞受精及胚胎质量方面的作用。
在47份连续采集的卵泡液中测量瘦素浓度，这些卵泡液是在取卵时发现有成熟卵母细胞的。将受精卵母细胞发育而来的胚胎进行质量评分。
在16 - 18小时检查时，卵母细胞显示有2个原核的卵泡液样本（25份）中的瘦素平均浓度显著高于未受精证据的样本（22份）（分别为26.0±6.1与15.3±10.6 ng/ml，p<0.01）。两组卵泡平均直径相似（分别为21.4±3.4和21.0±5.1 mm）。逻辑回归分析确定卵泡液瘦素水平是卵母细胞受精的最佳预测参数（p<0.001）。当采用接受操作特征曲线时，卵泡液瘦素浓度20.25 ng/ml是预测卵母细胞受精最可靠的临界值。瘦素浓度高于此值的卵泡液样本（27份）的卵母细胞受精率为85.7%。相比之下，卵泡液水平≤20.25 ng/ml的样本（20份）受精率为16.7%（p<0.05）。卵泡液瘦素与15个有价值胚胎在合子期（r = -0.01）及授精后48小时（r = 0.1）的评分之间无相关性。
卵泡液瘦素水平比卵泡直径更能准确预测卵母细胞受精成功率。这些结果强调了卵泡液变量在开发卵母细胞选择方法中的重要性。
