Drug Applied Research Center, Tabriz University of Medical Sciences, Tabriz, Iran; Student Research Committee, Tabriz University of Medical Sciences, Tabriz, Iran.
Immunology Research Center, Tabriz University of Medical Sciences, Tabriz, Iran.
Biomed Pharmacother. 2017 Sep;93:737-745. doi: 10.1016/j.biopha.2017.07.011. Epub 2017 Jul 9.
Nowadays, all people are at risk of infectious diseases that are mainly caused by bacteria causing infection. There is a permanent demand for an appropriate detection method that is affordable, practical, careful, rapid, sensitive, efficient and economical. Aptamers are single stranded DNA or RNA oligonucleotides, which can be recognized specifically and bind to their target molecules and also, be exploited in diagnostic applications. Recently, aptamer-based systems have offered great potentials in applications for the recognition of several important bacterial pathogens from clinical and food specimens. There are several reports appraising the diagnostic applicability of aptamer-based systems for the detection of pathogens. As for its excellent sensitivity, as well as its rapid and efficient detectability, this technique may be practical to indicate bacterial targets with less sample size and may consume less time than traditional methods These systems offer a promising approach for the sensitive and quick detection of food-borne and clinical agents. This review provides an overview of aptamer-based methods as a novel approach for detecting bacterial pathogens.
如今，所有人都面临着由细菌引起的传染病的风险。因此，我们需要一种价格合理、实用、谨慎、快速、敏感、高效和经济的合适检测方法。适体是单链 DNA 或 RNA 寡核苷酸，能够特异性识别并与目标分子结合，也可用于诊断应用。最近，基于适体的系统在从临床和食品样本中识别几种重要的细菌病原体的应用中展现出了巨大的潜力。有一些报告评估了基于适体的系统在检测病原体方面的诊断适用性。这种技术具有出色的灵敏度以及快速高效的检测能力，可能适用于检测具有较少样本量的细菌靶标，并且比传统方法消耗的时间更少。这些系统为快速灵敏地检测食源性和临床制剂提供了很有前景的方法。本文综述了基于适体的方法作为一种新型的细菌病原体检测方法。
