Characterization of RUNX1T1, an Adipogenesis Regulator in Ovine Preadipocyte Differentiation.

Runt-related transcription factor 1 translocation partner 1 (RUNX1T1), a potential novel regulator of adipogenesis, exists in two splice variants: a long (RUNX1T1-L) and a short (RUNX1T1-S) isoform. However, there is no data showing the existence of in ovine subcutaneous fat at different stages of developmental and its role on ovine adipogenesis. Therefore, the objectives of this study were to evaluate the presence of in subcutaneous fat of five-day-old to 24-month-old sheep and to investigate the role of in ovine adipogenesis. In this study, we detected a 1829 bp cDNA fragment of which contains a 1815 bp coding sequence that encodes 602-amino acid and 14 bp of 5' untranslated region, respectively. The amino acid sequence of RUNX1T1 has 31.18⁻94.21% homology with other species' protein sequences. During fat development, the RUNX1T1 protein expression was higher in subcutaneous fat of 24-month-old Hu sheep. In addition, the expression of mRNA decreased first, then subsequently increased during ovine preadipocyte differentiation. Knockdown of in ovine preadipocytes promoted preadipocyte differentiation and lipid accumulation. Taken together, our data suggests that is an important functional molecule in adipogenesis. Moreover, it showed for the first time that was negatively correlated with the ovine preadipocyte differentiation.