Zolfaghari Sina, Soleimanzadeh Ali, Baqerkhani Mohammadreza
Department of Theriogenology, Faculty of Veterinary Medicine, Urmia University, Urmia, Iran.
DVM Graduate, Faculty of Veterinary Medicine, Urmia University, Urmia, Iran.
Sci Rep. 2025 Apr 8;15(1):12053. doi: 10.1038/s41598-025-96413-9.
This study examines a potential treatment for testicular ischemia-reperfusion (I/R) injury using fisetin (FIS) and quercetin (QUE) in a rat model. Male rats were divided into five groups: a control group, a torsion/detorsion (T/D) group, and three experimental groups treated with FIS, QUE, or a combination of FIS + QUE. Sperm parameters, oxidative stress markers, histopathological features, RT-PCR analysis of apoptotic and antiapoptotic gene expression, and fertility index were evaluated. The results demonstrated that FIS + QUE, FIS, and QUE significantly improved sperm motility and concentration, leading to a higher fertility index, than the reduced metrics in the T/D group. Additionally, levels of MDA and NO were significantly lowered, while CAT, SOD, GPx, and TAC levels increased in the FIS + QUE, FIS, and QUE groups. Histopathological, RT-PCR and fertility analyses also revealed evidence of apoptosis and testicular damage in the T/D group, shown by significant increases in P53, Bax, and caspase-3, along with marked decreases in AKT, PI3K, and Bcl-2. Treatment with FIS and QUE, particularly in combination, significantly improved outcomes, indicating a strong synergistic effect that helps repair damage and enhance reproductive function after T/D injury.
本研究在大鼠模型中研究了使用非瑟酮（FIS）和槲皮素（QUE）对睾丸缺血再灌注（I/R）损伤的一种潜在治疗方法。雄性大鼠分为五组：对照组、扭转/去扭转（T/D）组以及用FIS、QUE或FIS + QUE组合处理的三个实验组。评估了精子参数、氧化应激标志物、组织病理学特征、凋亡和抗凋亡基因表达的RT-PCR分析以及生育指数。结果表明，与T/D组降低的指标相比，FIS + QUE、FIS和QUE显著改善了精子活力和浓度，导致更高的生育指数。此外，FIS + QUE、FIS和QUE组中丙二醛（MDA）和一氧化氮（NO）水平显著降低，而过氧化氢酶（CAT）、超氧化物歧化酶（SOD）、谷胱甘肽过氧化物酶（GPx）和总抗氧化能力（TAC）水平升高。组织病理学、RT-PCR和生育分析还显示T/D组存在细胞凋亡和睾丸损伤的证据，表现为P53、Bax和半胱天冬酶-3显著增加，同时AKT、磷脂酰肌醇-3激酶（PI3K）和Bcl-2显著降低。用FIS和QUE治疗，尤其是联合使用，显著改善了结果，表明有强大的协同作用，有助于修复T/D损伤后的损伤并增强生殖功能。
