Department of Dermatology, Shengjing Hospital of China Medical University, Heping District, Shenyang, Liaoning 110004, China.
Department of Dermatology, Shengjing Hospital of China Medical University, Heping District, Shenyang, Liaoning 110004, China.
Int Immunopharmacol. 2018 Jul;60:9-17. doi: 10.1016/j.intimp.2018.04.029. Epub 2018 Apr 24.
Atopic dermatitis (AD) is a chronic relapsing inflammatory skin disease prevalent worldwide. This study investigated the effects of glycyrrhizin, an extract of licorice root, on the well-established model of 2,4-dinitrochlorobenzene-induced AD-like symptoms in mice. The severity of dermatitis, histopathological changes, serum IgE levels, changes in expression of high-mobility group box 1 (HMGB1), the receptor for advanced glycation end products (RAGE), nuclear factor (NF)-κB and inflammatory cytokines were evaluated. Treatment with glycyrrhizin inhibited the HMGB1 signaling cascade and ameliorated the symptoms of AD. Furthermore, in an in vitro study, the expression of RAGE was detected in a mouse mast cell line, P815 cells, and rmHMGB1 was found to be a potent inducer of mast cell activation by increasing Ca influx, upregulating the CD117 and activating NF-κB signaling; these effects were also inhibited by glycyrrhizin. These findings implicate HMGB1 in the pathogenesis of AD and suggest that GL could be an effective therapeutic approach for cutaneous inflammation.
特应性皮炎（AD）是一种在全球范围内普遍存在的慢性复发性炎症性皮肤病。本研究探讨了甘草酸苷（甘草根的提取物）对已建立的 2,4-二硝基氯苯诱导的 AD 样症状小鼠模型的影响。评估了皮炎的严重程度、组织病理学变化、血清 IgE 水平、高迁移率族蛋白 B1（HMGB1）、晚期糖基化终产物受体（RAGE）、核因子（NF）-κB 和炎症细胞因子表达的变化。甘草酸苷治疗抑制了 HMGB1 信号级联反应，改善了 AD 的症状。此外，在体外研究中，在小鼠肥大细胞瘤系 P815 细胞中检测到 RAGE 的表达，并且发现 rmHMGB1 通过增加 Ca2+内流、上调 CD117 和激活 NF-κB 信号来强烈诱导肥大细胞活化；这些作用也被甘草酸苷抑制。这些发现表明 HMGB1 参与了 AD 的发病机制，并提示 GL 可能是治疗皮肤炎症的有效方法。
