Centre of Advanced Study in Botany, Institute of Science, Banaras Hindu University, Varanasi 221005, India.
Centre of Advanced Study in Botany, Institute of Science, Banaras Hindu University, Varanasi 221005, India.
Bioresour Technol. 2018 Aug;262:124-131. doi: 10.1016/j.biortech.2018.04.067. Epub 2018 Apr 19.
The aim of this work was to study cellulose degradation and whole genome sequence of Paenibacillus lautus BHU3 isolate. The 16S rRNA gene sequence analysis revealed genetic relatedness (99%) of Iso 7 with Paenibacillus lautus, Iso 8 with Paenibacillus lactis, and Iso 9 with Bacillus amyloliquefaciens. Clear zone formation followed by CMCase and FPase assays exhibited cellulolytic potential in the order: P. lautus > P. lactis > B. amyloliquefaciens. The most potent isolate, Paenibacillus lautus strain BHU3 was subjected to whole genome analysis with reference to the genomic basis of cellulose degradation. Results showed that P. lautus strain BHU3 contains 6234 protein coding genes of which, 316 were associated with the carbohydrate metabolism. Further, genomic CAZymes analysis indicated that the P. lautus strain BHU3 comprising a range of glycoside hydrolase (GH) family genes (143), may play the vital role(s) in enhancing the cellulolytic attributes, and could be the useful tool for lignocellulosic biomass degradation and waste management.
本工作旨在研究解淀粉芽胞杆菌 BHU3 分离株的纤维素降解和全基因组序列。16S rRNA 基因序列分析显示 Iso7 与解淀粉芽胞杆菌具有遗传相关性（99%），Iso8 与乳酸芽胞杆菌具有遗传相关性，Iso9 与解淀粉芽胞杆菌具有遗传相关性。透明圈形成后进行 CMCase 和 FPase 测定，显示纤维素降解活性依次为：解淀粉芽胞杆菌>乳酸芽胞杆菌>解淀粉芽胞杆菌。具有最强活性的分离株解淀粉芽胞杆菌 BHU3 进行了全基因组分析，以参考纤维素降解的基因组基础。结果表明，解淀粉芽胞杆菌 BHU3 含有 6234 个蛋白质编码基因，其中 316 个与碳水化合物代谢有关。此外，基因组 CAZymes 分析表明，解淀粉芽胞杆菌 BHU3 包含一系列糖苷水解酶（GH）家族基因（143 个），可能在增强纤维素酶特性方面发挥重要作用，并且可能是木质纤维素生物质降解和废物管理的有用工具。
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