State Key Laboratory for Agrobiotechnology, Department of Microbiology and Immunology, College of Biological Sciences, China Agricultural University, Beijing, 100193, People's Republic of China.
Antonie Van Leeuwenhoek. 2012 Nov;102(4):735-41. doi: 10.1007/s10482-012-9773-4. Epub 2012 Jul 12.
A nitrogen-fixing bacterium, designated strain gs65(T), was isolated from a rhizosphere soil sample of Caragana kansuensis Pojark. Phylogenetic analysis based on a fragment of the nifH gene and the full-length 16S rRNA gene sequence revealed that strain gs65(T) is a member of the genus Paenibacillus. High levels of 16S rRNA gene similarity were found between strain gs65(T) and Paenibacillus borealis DSM 13188(T) (97.5 %), Paenibacillus odorifer ATCC BAA-93(T) (97.3 %), Paenibacillus durus DSM 1735(T) (97.0 %) and Paenibacillus sophorae DSM23020(T) (96.9 %). Levels of 16S rRNA gene sequence similarity between strain gs65(T) and the type strains of other recognized members of the genus Paenibacillus were below 97.0 %. Levels of DNA-DNA relatedness between strain gs65(T) and P. borealis DSM 13188(T), P. odorifer ATCC BAA-93(T) (97.3 %), P. durus DSM 1735(T) and P. sophorae DSM23020(T) were 35.9, 38.0, 34.2 and 35.5 % respectively. The DNA G+C content of strain gs65(T) was determined to be 51.6 mol%. The major fatty acids were found to be iso-C(14:0), anteiso-C(15:0) and iso-C(16:0). On the basis of its phenotypic characteristics and levels of DNA-DNA hybridization, strain gs65(T) is considered to represent a novel species of the genus Paenibacillus, for which the name Paenibacillus taohuashanense sp. nov. is proposed. The type strain is gs65(T) (=CGMCC 1.12175(T) = DSM 25809(T)).
一株固氮菌，命名为 gs65(T) 菌株，从胡枝子根际土壤样本中分离得到。基于 nifH 基因片段和 16S rRNA 全序列的系统发育分析表明，gs65(T) 菌株是芽孢杆菌属的一个成员。gs65(T) 菌株与解淀粉芽孢杆菌 DSM 13188(T)（97.5%）、恶臭芽孢杆菌 ATCC BAA-93(T)（97.3%）、坚韧芽孢杆菌 DSM 1735(T)（97.0%）和槐糖脂芽孢杆菌 DSM23020(T)（96.9%）的 16S rRNA 基因相似度很高。gs65(T) 菌株与其他已鉴定的芽孢杆菌属成员的模式菌株的 16S rRNA 基因序列相似度均低于 97.0%。gs65(T) 菌株与解淀粉芽孢杆菌 DSM 13188(T)、恶臭芽孢杆菌 ATCC BAA-93(T)（97.3%）、坚韧芽孢杆菌 DSM 1735(T)和槐糖脂芽孢杆菌 DSM23020(T)之间的 DNA-DNA 同源性分别为 35.9%、38.0%、34.2%和 35.5%。gs65(T) 菌株的 DNA G+C 含量为 51.6mol%。主要脂肪酸为 iso-C(14:0)、anteiso-C(15:0)和 iso-C(16:0)。基于其表型特征和 DNA-DNA 杂交水平，gs65(T) 菌株被认为代表了芽孢杆菌属的一个新种，现命名为桃华山芽孢杆菌新种。模式菌株为 gs65(T)=CGMCC 1.12175(T)=DSM 25809(T)。
