Department of Operative Dentistry and Endodontics, School and Hospital of Stomatology, Tongji University, Shanghai Engineering Research Center of Tooth Restoration and Regeneration, Shanghai, China.
Department of Operative Dentistry and Endodontics, School and Hospital of Stomatology, Tongji University, Shanghai Engineering Research Center of Tooth Restoration and Regeneration, Shanghai, China.
J Endod. 2018 Jun;44(6):977-983. doi: 10.1016/j.joen.2018.03.006. Epub 2018 Apr 24.
Concentrated growth factor (CGF) is considered to be a natural biomaterial that is better than platelet-rich fibrin (PRF) in bone regeneration, but there is little information acquired in regenerative endodontics. Therefore, the purpose of this study was to evaluate their effects on the proliferation, migration, and differentiation of human stem cells of the apical papilla (SCAPs).
CGF- and PRF-conditioned medium were prepared using the freeze-dried method. SCAPs were isolated and identified. The proliferative potential of SCAPs was investigated using the Cell Counting Kit-8 (KeyGen Biotech, Nanjing, China). The migration capacity was analyzed using transwell assays, and the mineralization ability was determined by alizarin red S staining. The expression levels of alkaline phosphatase, bone sialoprotein, dentin matrix protein 1, and dentin sialophosphoprotein were determined by quantitative polymerase chain reaction.
The cultured cells exhibited mesenchymal stem cell characteristics. The growth rate and migratory cell numbers of the CGF and PRF groups were significantly greater than those of the control group. The mineralized areas in the CGF and PRF groups were significantly larger than those in the control group after incubation for 7 days and 14 days. The expression levels of osteogenic/odontoblast-related genes were reduced on day 7, but they were dramatically enhanced on day 14, and the related gene expression levels in the PRF group were higher than those in the CGF group.
Both CGF and PRF can promote the proliferation, migration, and differentiation of SCAPs. CGF may be a promising alternative in regenerative endodontics.
浓缩生长因子（CGF）被认为是一种优于富血小板纤维蛋白（PRF）的天然生物材料，在骨再生方面效果更好，但在再生牙髓学方面的信息较少。因此，本研究旨在评估其对根尖乳头干细胞（SCAPs）增殖、迁移和分化的影响。
采用冻干法制备 CGF 和 PRF 条件培养基。分离并鉴定 SCAPs。使用细胞计数试剂盒-8（KeyGen Biotech，南京，中国）检测 SCAPs 的增殖潜力。通过 Transwell 测定分析迁移能力，通过茜素红 S 染色测定矿化能力。通过定量聚合酶链反应测定碱性磷酸酶、骨涎蛋白、牙本质基质蛋白 1 和牙本质涎磷蛋白的表达水平。
培养的细胞表现出间充质干细胞的特征。CGF 和 PRF 组的生长速度和迁移细胞数明显大于对照组。孵育 7 天和 14 天后，CGF 和 PRF 组的矿化面积明显大于对照组。成骨/成牙本质相关基因的表达水平在第 7 天降低，但在第 14 天显著增强，PRF 组的相关基因表达水平高于 CGF 组。
CGF 和 PRF 均可促进 SCAPs 的增殖、迁移和分化。CGF 可能是再生牙髓学中一种有前途的替代方法。
