Department of Pediatric Dentistry, Faculty of Dentistry, Recep Tayyip Erdogan University, Rize, 53100, Turkey.
Department of Endodontics, Faculty of Dentistry, Recep Tayyip Erdogan University, Rize, 53100, Turkey.
BMC Oral Health. 2024 Aug 3;24(1):890. doi: 10.1186/s12903-024-04577-z.
This study aims to investigate the effect of Mineral Trioxide Aggregate (MTA), a bioactive endodontic cement, and Concentrated Growth Factor (CGF), a second-generation autologous growth factor, on pulpotomy-induced pulp inflammation. The study utilized the maxillary anterior central teeth of thirty-six young male Sprague Dawley rats. Forty-eight teeth were randomly assigned to two groups (12 rats/group; 24 teeth/group) based on the capping material (MTA or CGF). Subsequently, two subgroups (MTAG and CGFG) were formed per group (12 teeth/group) based on the time following pulpotomy (2-weeks and 4-weeks). The central teeth of the 12 animals assigned to the control group (CG) were not manipulated in any way, both in the 2-week group and in the 4-week group. Tissue samples extracted from rats at the end of the experiment were stained with H&E for histopathological analysis. For immunohistochemical analysis, primary antibodies for TNF-α and NF-kβ/65 were incubated. Data obtained from semi-quantitative analysis were assessed for normal distribution using Skewness-Kurtosis values, Q-Q plot, Levene's test, and the Shapiro-Wilk test on statistical software. A P value < 0.05 was considered significant. When compared with the control group, both MTAG and CGFG showed increased edematous and inflammatory areas. In MTAG, edematous and inflammatory areas decreased significantly from the 2nd week (2(2-2), 2(1-2)) to the 4th week (1(1-1), 1(0-1)), while in CGFG, edematous areas decreased (2(2-3), 1.5(1-2)), and inflammatory areas increased significantly (2(2-3), 3(2-2.5)). When compared with the control group, TNF-α and NF-kβ/p65 positivity were higher in both MTAG and CGFG. In MTAG, TNF-α [2(1.5-2)] and NF-kβ/p65 [1.5(1-2)] positivity decreased significantly from the 2nd week to the 4th week [TNF-α: 1(1-1), NF-kβ/p65: 1(1-2)], while no significant change was observed in CGFG. In conclusion, this study revealed a reduction in cells showing TNF-α and NF-kβ/p65 positivity in the MTA treatment group compared to the CGF group. Although MTA demonstrated more favorable results than CGF in mitigating pulpal inflammation within the scope of this study, further experimental and clinical investigations are warranted to obtain comprehensive data regarding CGF.
本研究旨在探讨矿物三氧化物聚合体（MTA）这一生物活性根管封闭剂和浓缩生长因子（CGF）这一二代自体生长因子对活髓切断术诱导的牙髓炎症的影响。研究采用 36 只雄性 Sprague Dawley 大鼠的上颌前中切牙。48 颗牙齿根据封闭材料（MTA 或 CGF）随机分为两组（每组 12 只大鼠；每组 24 颗牙齿）。随后，每组根据活髓切断术后时间（2 周和 4 周）分为两组（每组 12 颗牙齿）（MTAG 和 CGFG）。分配给对照组（CG）的 12 只动物的中央牙齿在 2 周组和 4 周组中均未进行任何操作。实验结束时从大鼠中提取组织样本，用 H&E 染色进行组织病理学分析。为进行免疫组织化学分析，孵育 TNF-α 和 NF-kβ/65 的一抗。使用偏度-峰度值、Q-Q 图、Levene 检验和 Shapiro-Wilk 检验在统计软件上评估半定量分析获得的数据是否符合正态分布。P 值<0.05 被认为具有统计学意义。与对照组相比，MTAG 和 CGFG 均显示出水肿和炎症区域增加。在 MTAG 中，从第 2 周（2(2-2)，2(1-2)）到第 4 周（1(1-1)，1(0-1)），水肿和炎症区域显著减少，而在 CGFG 中，水肿区域减少（2(2-3)，1.5(1-2)），炎症区域显著增加（2(2-3)，3(2-2.5)）。与对照组相比，MTAG 和 CGFG 中 TNF-α 和 NF-kβ/p65 的阳性率均更高。在 MTAG 中，TNF-α[2(1.5-2)]和 NF-kβ/p65[1.5(1-2)]阳性率从第 2 周到第 4 周显著降低[TNF-α：1(1-1)，NF-kβ/p65：1(1-2)]，而 CGFG 中未观察到显著变化。总之，与 CGF 组相比，MTA 治疗组中显示 TNF-α 和 NF-kβ/p65 阳性的细胞减少。尽管在本研究范围内，MTA 对减轻牙髓炎症的效果优于 CGF，但需要进一步的实验和临床研究来获得关于 CGF 的全面数据。
