Turner Andrew D, Broadwater Maggie, Van Dolah Frances
Centre for Environment Fisheries and Aquaculture Science, Barrack Road, Weymouth, Dorset, DT4 8UB, UK.
National Oceanic and Atmospheric Administration, National Centers for Coastal Ocean Science, Stressor Detection and Impacts Division, Charleston, SC, USA.
Toxicon. 2018 Jun 15;148:155-164. doi: 10.1016/j.toxicon.2018.04.025. Epub 2018 Apr 26.
A receptor binding assay (RBA) for the determination of paralytic shellfish poisoning toxicity is formally validated through collaborative study and approved for regulatory monitoring use in the US for mussels and clams. However, to date, the method has not been tested on bivalve molluscs originating from European waters and no validation studies have been conducted for oysters, a shellfish species of great importance globally. This study firstly reports the work conducted to assess the performance of the assay in comparison with a regulatory chemical detection method for a range of shellfish species originating from Great Britain. Data obtained showed a complete absence of false negative RBA results, with a tendency to over-estimate PSP toxicity for some shellfish species in comparison with liquid chromatography with fluorescence detection. Secondly, the performance of the RBA was assessed for oysters, with the analysis of a dilution series of oyster matrix certified reference materials. Method trueness, sensitivity and precision were found to compare well with results reported previously for other species. In addition, the RBA analysis of untreated and demetallated oyster extracts, provided good evidence that the RBA is not suppressed in the presence of high concentrations of zinc as reported previously for the mouse bioassay. Consequently, there is strong evidence from this study, that the RBA would be suitable for determination of PSP toxicity in bivalve molluscs from GB, with acceptable method performance in oysters. Further validation studies would be required for other shellfish species of interest before the method can be considered suitable for implementation in Europe.
一种用于测定麻痹性贝类中毒毒性的受体结合测定法（RBA）已通过协作研究得到正式验证，并在美国被批准用于贻贝和蛤的监管监测。然而，迄今为止，该方法尚未在源自欧洲水域的双壳贝类上进行测试，也没有针对牡蛎（一种在全球具有重要意义的贝类物种）开展验证研究。本研究首次报告了为评估该测定法与一种用于检测源自英国的一系列贝类物种的监管化学检测方法相比的性能而开展的工作。所获得的数据显示完全没有RBA假阴性结果，与荧光检测液相色谱法相比，某些贝类物种的麻痹性贝类中毒毒性存在被高估的趋势。其次，通过分析一系列稀释的牡蛎基质有证标准物质，对牡蛎的RBA性能进行了评估。发现该方法的准确性、灵敏度和精密度与先前报道的其他物种的结果相当。此外，对未经处理和脱金属的牡蛎提取物进行的RBA分析提供了有力证据，证明RBA在高浓度锌存在的情况下不会像先前小鼠生物测定法那样受到抑制。因此，本研究有充分证据表明，RBA适用于测定源自英国的双壳贝类中的麻痹性贝类中毒毒性，在牡蛎中具有可接受的方法性能。在该方法被认为适合在欧洲实施之前，还需要对其他感兴趣的贝类物种进行进一步的验证研究。
