State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing, 100193, China.
College of Life and Environmental Science, Penryn campus, University of Exeter, Penryn, TR10 9FE, UK.
Microbiol Res. 2018 Jun;211:13-20. doi: 10.1016/j.micres.2018.02.008. Epub 2018 Mar 23.
Rhizosphere microorganisms contribute to the health and development of crops and these beneficial microbes are recruited to the root-zone when plants experience biotic/abiotic stress. The subterranean pests Holotrichia parallela cause severe crop loss in peanut (Arachis hypogaea L.) fields. Hypothesizing that infestation by H. parallela larva may influence the composition of rhizosphere microbial communities, deep sequencing of V3 and V4 hypervariable regions of 16S rRNA gene was used to characterize the rhizosphere bacteria of infested and uninfested peanuts. A total of 2,673,656 reads were generated and an average of 2558 OTUs were obtained for each sample. Comparisons of rhizosphere bacterial community structure of peanuts with those infested by H. parallela larva revealed that the relative abundance of Proteobacteria and Bacteroidetes increased, while that of Actinobacteria decreased in the rhizosphere with infestation. A significant shift in bacterial communities was observed within 24 h after infestation by principal coordinate analysis. For the 332 genera identified in 24 h treatment, infestation of white grubs led to the significant changes of abundance of 67 genera. An increase in the Pseudomonas genus of infested-samples for 24 h was verified by real-time qPCR. Our results indicate H. parallela larvae infestation can quickly leads to the change of peanut rhizosphere microbiome and enrichment of specific bacterial species. But the effects were not persistent. This study provides the insight into the function of rhizosphere microbiome in the interaction between subterranean pests and crops.
根际微生物有助于作物的健康和生长发育，当植物受到生物/非生物胁迫时，这些有益微生物会被招募到根区。金针虫 Holotrichia parallela 是花生（Arachis hypogaea L.）田中严重的作物害虫。推测幼虫的侵害可能会影响根际微生物群落的组成，因此使用 V3 和 V4 高变区 16S rRNA 基因的深度测序来描述受侵染和未受侵染的花生的根际细菌。共产生了 2673656 个reads，每个样品的平均 OTU 数为 2558 个。比较受 H. parallela 幼虫侵染和未受侵染的花生根际细菌群落结构发现，根际中变形菌门和拟杆菌门的相对丰度增加，而放线菌门的相对丰度减少。主坐标分析显示，在幼虫侵染后 24 小时内观察到细菌群落发生了显著变化。在 24 小时处理中鉴定出的 332 个属中，有 67 个属的丰度因白土蚕的侵染而发生显著变化。实时 qPCR 验证了侵染样本中假单胞菌属的丰度增加。研究结果表明，幼虫的侵染会迅速导致花生根际微生物组的变化和特定细菌物种的富集。但这种影响不是持久的。本研究为根际微生物组在地下害虫与作物相互作用中的功能提供了新的认识。
