Shandong Peanut Research Institute, Shandong Academy of Agricultural Sciences, Qingdao 266100, China.
College of Plant Protection, Hebei Agricultural University, Baoding 071001, China.
Int J Mol Sci. 2020 Mar 20;21(6):2131. doi: 10.3390/ijms21062131.
Exposure of seeds to high salinity can cause reduced germination and poor seedling establishment. Improving the salt tolerance of peanut ( L.) seeds during germination is an important breeding goal of the peanut industry. Bacterial communities in the spermosphere soils may be of special importance to seed germination under salt stress, whereas extant results in oilseed crop peanut are scarce.
Here, bacterial communities colonizing peanut seeds with salt stress were characterized using 16S rRNA gene sequencing.
Peanut spermosphere was composed of four dominant genera: , , , and . Comparisons of bacterial community structure revealed that the beneficial bacteria (), which can produce specific phosphatases to sequentially mineralize organic phosphorus into inorganic phosphorus, occurred in relatively higher abundance in salt-treated spermosphere soils. Further soil enzyme activity assays showed that phosphatase activity increased in salt-treated spermosphere soils, which may be associated with the shift of .
This study will form the foundation for future improvement of salt tolerance of peanuts at the seed germination stage via modification of the soil microbes.
种子暴露在高盐环境下会导致发芽率降低和幼苗成活率低。提高花生种子在发芽过程中的耐盐性是花生产业的一个重要育种目标。种皮土壤中的细菌群落可能对盐胁迫下的种子发芽具有特殊意义，而现有的含油作物花生的研究结果却很少。
本研究使用 16S rRNA 基因测序技术，对盐胁迫下花生种子定殖的细菌群落进行了表征。
花生种皮由四个主要属组成：、、、和。细菌群落结构的比较表明，有益细菌（）在盐处理的种皮土壤中相对丰度较高，它们可以产生特定的磷酸酶，将有机磷依次矿化为无机磷。进一步的土壤酶活性测定表明，盐处理的种皮土壤中的磷酸酶活性增加，这可能与的变化有关。
本研究将为未来通过改良土壤微生物来提高花生种子发芽阶段的耐盐性奠定基础。
