State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing, China.
State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing, China; College of Life Sciences, Northeast Agricultural University, Harbin, China.
Microbiol Res. 2024 Apr;281:127597. doi: 10.1016/j.micres.2023.127597. Epub 2024 Jan 11.
Pest feeding affects the rhizobacteria community. The rhizomicrobiota activates salicylic acid and jasmonic acid signaling pathways to help plants deal with pest infestation. However, whether plants can recruit special pesticidal microorganisms to deal with attack from herbivores is unclear. A system composed of peanuts and first-instar larvae of Holotrichia parallela were used to analyze whether peanuts truly enrich the insecticidal bacteria after feeding by larvae, and whether inoculation of the enriched bacteria promotes the resistance of plants to herbivore. In this study, high-throughput sequencing of 16 S rRNA gene amplicons was used to demonstrate that infestation of the subterranean pest H. parallela quickly changed the rhizosphere bacterial community structure within 24 h, and the abundance of Enterobacteriaceae, especially Enterobacter, was manifestly enriched. Root feeding induced rhizobacteria to form a more complex co-occurrence network than the control. Rhizosphere bacteria were isolated, and 4 isolates with high toxicity against H. parallela larvae were obtained by random forest analysis. In a back-inoculation experiment using a split-root system, green fluorescent protein (GFP)-labeled Enterobacter sp. IPPBiotE33 was observed to be enriched in uneaten peanut roots. Additionally, supplementation with IPPBiotE33 alleviated the adverse effects of H. parallela on peanuts. Our findings indicated that herbivore infestation could induce plants to assemble bacteria with specific larvicidal activity to address threats.
取食会影响根际细菌群落。根际微生物会激活水杨酸和茉莉酸信号通路，帮助植物应对虫害。然而，植物是否能够招募特殊的杀虫微生物来应对食草动物的攻击尚不清楚。本研究构建了一个由花生和暗黑鳃金龟幼虫组成的系统，用于分析幼虫取食后花生是否真的富集了杀虫细菌，以及接种富集细菌是否能促进植物对食草动物的抗性。在这项研究中，通过高通量测序 16S rRNA 基因扩增子来证明，地下害虫暗黑鳃金龟的取食在 24 小时内迅速改变了根际细菌群落结构，肠杆菌科，特别是肠杆菌的丰度明显富集。根际取食诱导根际细菌形成比对照更复杂的共现网络。从根际分离细菌，通过随机森林分析获得了对暗黑鳃金龟幼虫具有高毒性的 4 个分离株。在使用分根系统的回接种实验中，观察到绿色荧光蛋白（GFP）标记的肠杆菌 IPPBiotE33 在未被取食的花生根中富集。此外，补充 IPPBiotE33 缓解了暗黑鳃金龟对花生的不利影响。研究结果表明，取食会诱导植物组装具有特定杀虫活性的细菌来应对威胁。
