Thin Film Materials Laboratory, Department of Physics, Shivaji University, Kolhapur, MS, India.
Department of Chemistry, The New College, Shivaji University, Kolhapur, MS, India.
Colloids Surf B Biointerfaces. 2018 Jul 1;167:425-431. doi: 10.1016/j.colsurfb.2018.04.042. Epub 2018 Apr 22.
To utilize synergetic effect of graphene's higher conductivity and magnetic nanoparticles biocompatibility, an electrochemical nanobiosensor is constructed based on magnetic nanoparticle decorated graphene (MRGO) using Horseradish peroxidase (HRP) for HO sensing. Sensors based on magnetic nanoparticles (MNP) and reduced graphene oxide (RGO) are studied for comparison. MNP, RGO and MRGO were characterized by X-ray diffraction (XRD), Transmission electron microscopy (TEM) and Fourier transform infrared spectroscopy (FTIR). XRD studies have confirmed successful synthesis of FeO MNPs, RGO and MRGO. TEM micrographs revealed uniform decoration of MNPs on graphene. FTIR confirmed the immobilization of HRP on MNP, RGO and MRGO. The MRGO based sensor exhibited higher sensitivity (48.08 μA μM cm) compared to MNP (39.08 μA μM cm) and RGO (41.08 μA μM cm) based biosensors.
为了利用石墨烯的高导电性和磁性纳米粒子的生物相容性的协同效应，基于磁性纳米粒子修饰的石墨烯（MRGO）构建了一种电化学纳米生物传感器，该传感器用于 HO 传感的辣根过氧化物酶（HRP）。对基于磁性纳米粒子（MNP）和还原氧化石墨烯（RGO）的传感器进行了比较研究。通过 X 射线衍射（XRD）、透射电子显微镜（TEM）和傅里叶变换红外光谱（FTIR）对 MNP、RGO 和 MRGO 进行了表征。XRD 研究证实了 FeO MNPs、RGO 和 MRGO 的成功合成。TEM 显微照片显示 MNPs 在石墨烯上均匀修饰。FTIR 证实了 HRP 在 MNP、RGO 和 MRGO 上的固定化。与基于 MNP（39.08 μA μM cm）和 RGO（41.08 μA μM cm）的生物传感器相比，基于 MRGO 的传感器表现出更高的灵敏度（48.08 μA μM cm）。
