Ito A, Takami T, Itoh M
Department of Parasitology, Gifu University School of Medicine, Japan.
Am J Trop Med Hyg. 1991 Jun;44(6):696-701. doi: 10.4269/ajtmh.1991.44.696.
Wistar rats inoculated intraperitoneally with 10 viable metacestodes of Taenia crassiceps without adjuvant once on day 0 showed strong resistance to challenge with 200 eggs of T. taeniaeformis on day 30. When rats were killed one month after challenge, there were 80.4% and 46.1% reductions in the number of cystic and total metacestodes of T. taeniaeformis in the liver, respectively. When five rats were killed 16 months after challenge, they showed almost complete immunity against the challenge, with 99.4% and 91.1% reductions in the number of cystic and total metacestodes, respectively. There were only a few degenerated, pin-point metacestodes of T. taeniaeformis in the liver of all five rats; one harbored one cystic metacestode as well. However, there were no such reductions in rats injected initially with cyst fluid antigens of T. crassiceps with Freund's complete adjuvant. An additional experiment was carried out using 500 eggs of T. taeniaeformis in order to confirm the vaccine effect against higher egg dose. There were 96.6%, 87.9%, 83.9%, and 79.3% reductions in the number of cystic metacestodes in rats initially inoculated with 10 viable, 10 formalized, and 10 frozen metacestodes, and injected with sodium deoxycholate-solubilized metacestode antigens, respectively. It is strongly suggested that rats singly dosed with 10 viable or non-viable, intact metacestodes of T. crassiceps without adjuvant became highly resistant to challenge infection with eggs of T. taeniaeformis, which resulted in almost no cystic metacestode establishment.
在第0天，Wistar大鼠腹腔内接种10个无佐剂的肥胖带绦虫活囊尾蚴，第30天对200个微小膜壳绦虫卵的攻击表现出强烈抗性。攻击后1个月处死大鼠时，肝脏中微小膜壳绦虫的囊尾蚴数量和总囊尾蚴数量分别减少了80.4%和46.1%。攻击后16个月处死5只大鼠时，它们表现出几乎完全的免疫力，囊尾蚴数量和总囊尾蚴数量分别减少了99.4%和91.1%。所有5只大鼠的肝脏中仅有少数退化的、针尖大小的微小膜壳绦虫囊尾蚴；其中1只还含有1个囊尾蚴。然而，最初用弗氏完全佐剂注射肥胖带绦虫囊液抗原的大鼠没有出现这种减少情况。为了确认疫苗对更高卵剂量的效果，进行了一项额外实验，使用500个微小膜壳绦虫卵。最初接种10个活的、10个甲醛固定的和10个冷冻的囊尾蚴，并注射脱氧胆酸钠溶解的囊尾蚴抗原的大鼠，其囊尾蚴数量分别减少了96.6%、87.9%、83.9%和79.3%。强烈建议，无佐剂单次接种10个肥胖带绦虫活的或非活的完整囊尾蚴的大鼠，对微小膜壳绦虫卵的攻击感染具有高度抗性，这导致几乎没有囊尾蚴形成。
