Ayala María E, Gonzáles Ayari, Olivarez Rodrigo M, Aragón-Martínez Andrés
Unidad de Investigación en en Biología de la Reproducción, Laboratorio de Pubertad, Facultad de Estudios Superiores Zaragoza, Universidad Nacional Autónoma de México (UNAM), AP 9-020, C.P. 15000, Distrito Federal, México.
Facultad de Estudios Superiores Iztacala, Universidad Nacional Autónoma de México (UNAM), Paseo de los Barrios Número 1, Los Reyes Iztacala, Tlalnepantla, Estado de México, C.P. 54090, México.
Reprod Fertil Dev. 2018 Oct;30(10):1329-1341. doi: 10.1071/RD17384.
Fluoxetine (Flx) is a selective serotonin reuptake inhibitor that alters the male reproductive system when administered at the adult stage or after maternal exposure. In the present study we evaluated the effects of Flx administration on reproductive parameters during juvenile-peripubertal development when treated male rats reached adulthood. Groups of rats were treated daily with Flx (5mgkg-1, i.p.) or saline (0.9% NaCl), or were left untreated. Rats were treated between 30 and 53 days of age and were killed at 65 days of age. Serotonin concentrations were determined in the hypothalamus, hypophysis and testis. Gonadotrophins, sex steroids and sperm quality (membrane integrity, sperm with functional mitochondria, sperm density, sperm motility and morphological abnormalities) were also evaluated. Flx did not affect bodyweight, but significantly diminished LH, FSH, progesterone and testosterone serum concentrations. After graphical analysis, a subgroup of rats was identified whose sperm quality parameters were greatly affected by Flx. In the present study we show that Flx administered to juvenile rats disrupts the hypothalamic-hypophyseal-testicular axis and its effects on sperm quality are not homogeneous in adults. In contrast, Flx altered concentrations of gonadotrophins and sexual steroids in all treated rats. These results suggest caution should be exercised in the prescription of Flx to prepubertal males.
氟西汀（Flx）是一种选择性5-羟色胺再摄取抑制剂，在成年期给药或母体接触后会改变雄性生殖系统。在本研究中，我们评估了在幼年-青春期发育期间给予氟西汀对生殖参数的影响，此时接受治疗的雄性大鼠已成年。将大鼠分组，每天给予氟西汀（5mg/kg，腹腔注射）或生理盐水（0.9%氯化钠），或不进行处理。大鼠在30至53日龄期间接受治疗，并在65日龄时处死。测定下丘脑、垂体和睾丸中的5-羟色胺浓度。还评估了促性腺激素、性类固醇和精子质量（膜完整性、具有功能性线粒体的精子、精子密度、精子活力和形态异常）。氟西汀不影响体重，但显著降低血清促黄体生成素（LH）、促卵泡生成素（FSH）、孕酮和睾酮浓度。经过图形分析，确定了一组大鼠，其精子质量参数受到氟西汀的极大影响。在本研究中，我们表明，给幼年大鼠施用氟西汀会破坏下丘脑-垂体-睾丸轴，并且其对成年大鼠精子质量的影响并不均匀。相比之下，氟西汀改变了所有接受治疗大鼠的促性腺激素和性类固醇浓度。这些结果表明，在给青春期前男性开氟西汀处方时应谨慎。
