Sun Yat-Sen Memorial Hospital, Sun Yat-Sen University, Guangzhou, China.
Weil Institute of Emergency and Critical Care Research, School of Medicine, Virginia Commonwealth University, Richmond, VA, USA.
Mediators Inflamm. 2018 Mar 7;2018:8352727. doi: 10.1155/2018/8352727. eCollection 2018.
We attempted to investigate whether blood lactate is a useful biomarker for sepsis in a rat cecal ligation and puncture (CLP) model. Male Sprague-Dawley rats underwent approximately 75% cecum ligation and two punctures to induce high-grade sepsis. A lactate of 1.64 mmol/L (Youden score of 0.722) was selected as the best cutoff value to predict the onset of sepsis after CLP exposure; 46 of 50 rats who survived 24 hours after the CLP were divided into the L group (lactate < 1.64 mmol/L) and M group (lactate ≥ 1.64 mmol/L). In the M group, the animals had significantly higher murine sepsis scores and none survived 5 days post-CLP, and the rate of validated septic animals, serum procalcitonin, high mobility group box 1, blood urea nitrogen, alanine transaminase, cardiac troponin I, and the wet-to-dry weight ratio were significantly higher compared to the L group. Worsen PaO/FiO, microcirculations, and mean arterial pressure were observed in the M group. More severe damage in major organs was confirmed by histopathological scores in the M group compared with the L group. In conclusion, lactate ≥ 1.64 mmol/L might serve as a potential biomarker to identify the onset of sepsis in a rat CLP model.
我们试图在大鼠盲肠结扎穿孔（CLP）模型中研究血乳酸是否可作为脓毒症的有用生物标志物。雄性 Sprague-Dawley 大鼠接受约 75%盲肠结扎和两次穿孔，以诱导重度脓毒症。选择 1.64mmol/L 的乳酸（约登指数为 0.722）作为最佳截断值，以预测 CLP 暴露后脓毒症的发生；在 CLP 后 24 小时存活的 50 只大鼠中，46 只被分为 L 组（乳酸＜1.64mmol/L）和 M 组（乳酸≥1.64mmol/L）。在 M 组，动物的鼠脓毒症评分明显更高，且无一只在 CLP 后 5 天存活，证实患有脓毒症的动物比例、血清降钙素原、高迁移率族蛋白 1、血尿素氮、丙氨酸氨基转移酶、心肌肌钙蛋白 I 和湿重/干重比明显高于 L 组。M 组的 PaO/FiO 恶化、微循环和平均动脉压均下降。M 组主要器官的组织病理学评分明显高于 L 组，证实了更严重的损伤。总之，乳酸≥1.64mmol/L 可能作为大鼠 CLP 模型中识别脓毒症发生的潜在生物标志物。
