Guizhou Medical University, Guiyang, Guizhou, China (mainland).
Department of Critical Care, Affiliated Hospital of Guizhou Medical University, Guiyang, Guizhou, China (mainland).
Med Sci Monit. 2020 Jan 29;26:e919054. doi: 10.12659/MSM.919054.
BACKGROUND The aim of this study was to evaluate the clinical characteristics of 2 rat models of sepsis for improved cecal ligation/puncture (CLP) and feces intraperitoneal-injection (FIP), including systemic inflammation, organ dysfunction, and blood coagulation. MATERIAL AND METHODS Sixty-two male SD rats were randomly divided into 3 groups: a normal control group (NC, n=6), a CLP group (n=28), and a FIP group (n=28). Ten rats each in the CLP and FIP groups were observed for 72-h mortality rate. The remaining 18 rats in each group were divided into 3 subgroups (n=6) according to their post-operation period (6, 12, and 24 h). Abdominal arterial blood was collected to determine the lactic acid (Lac) concentration, prothrombin time (PT), active partial prothrombin time (APTT), plasmic interleukin-6 (IL-6) level, and cardiac troponin (cTnI) level. The intestines, lung, and heart were collected for pathological examination. RESULTS The 72-h mortality rates in the CLP and FIP groups were 60% and 100%, respectively. The Lac level in both groups was significantly elevated at 6, 12, and 24 h after modeling. Compared with the NC group, PT in the CLP and FIP groups was prolonged at 12 and 24 h, and APTT was significantly prolonged at 6 h. IL-6 levels in the CLP and FIP groups peaked at 6 h. The cTnI level in the FIP group was significantly higher at 12 h after modeling compared with the NC group. The intestines, lung, and heart were pathologically damaged at 6 h, and this damage worsened over time. CONCLUSIONS Both modeling methods induced sepsis in rats and closely mimicked the clinical conditions, but FIP was easier to establish and was more suitable for standardization.
本研究旨在评估两种脓毒症大鼠模型（盲肠结扎穿孔术（CLP）和粪便腹腔内注射（FIP））的临床特征，包括全身炎症、器官功能障碍和血液凝血。
62 只雄性 SD 大鼠随机分为 3 组：正常对照组（NC，n=6）、CLP 组（n=28）和 FIP 组（n=28）。CLP 和 FIP 组各有 10 只大鼠观察 72 小时死亡率。每组其余 18 只大鼠根据术后时间（6、12 和 24 小时）分为 3 个亚组（n=6）。采集腹主动脉血测定乳酸（Lac）浓度、凝血酶原时间（PT）、活化部分凝血活酶时间（APTT）、血浆白细胞介素-6（IL-6）水平和心肌肌钙蛋白 I（cTnI）水平。收集肠、肺和心脏进行病理检查。
CLP 和 FIP 组的 72 小时死亡率分别为 60%和 100%。建模后 6、12 和 24 小时，两组 Lac 水平均明显升高。与 NC 组相比，CLP 和 FIP 组 PT 在 12 和 24 小时延长，APTT 在 6 小时明显延长。CLP 和 FIP 组 IL-6 水平在 6 小时达到峰值。FIP 组建模后 12 小时 cTnI 水平明显高于 NC 组。6 小时时肠、肺和心脏均有病理损伤，且随时间推移损伤加重。
两种建模方法均可诱导大鼠脓毒症，且与临床情况密切相关，但 FIP 法建立更简单，更适合标准化。
