Kaufmann Anton
TAM, Official Food Contol Authority of the Canton of Zurich, Fehrenstrasse, Zürich, Switzerland, 8030.
J Mass Spectrom. 2018 Aug;53(8):725-738. doi: 10.1002/jms.4195. Epub 2018 Jul 10.
Quadrupole Orbitrap instruments (Q Orbitrap) permit high-resolution mass spectrometry-based full scan acquisitions and have a number of acquisition modes where the quadrupole isolates a particular mass range prior to a possible fragmentation and high-resolution mass spectrometry-based acquisition. Selecting the proper acquisition mode(s) is essential if trace analytes are to be quantified in complex matrix extracts. Depending on the particular requirements, such as sensitivity, selectivity of detection, linear dynamic range, and speed of analysis, different acquisition modes may have to be chosen. This is particularly important in the field of multi-residue analysis (eg, pesticides or veterinary drugs in food samples) where a large number of analytes within a complex matrix have to be detected and reliably quantified. Meeting the specific detection and quantification performance criteria for every targeted compound may be challenging. It is the aim of this paper to describe the strengths and the limitations of the currently available Q Orbitrap acquisition modes. In addition, the incorporation of targeted acquisitions between full scan experiments is discussed. This approach is intended to integrate compounds that require an additional degree of sensitivity or selectivity into multi-residue methods.
四极杆-轨道阱质谱仪（Q Orbitrap）可进行基于高分辨率质谱的全扫描采集，并且有多种采集模式，在可能的碎裂和基于高分辨率质谱的采集之前，四极杆会隔离特定的质量范围。如果要在复杂基质提取物中对痕量分析物进行定量，选择合适的采集模式至关重要。根据特定要求，如灵敏度、检测选择性、线性动态范围和分析速度，可能必须选择不同的采集模式。这在多残留分析领域（例如食品样品中的农药或兽药）尤为重要，在该领域中，必须检测并可靠地定量复杂基质中的大量分析物。满足每种目标化合物的特定检测和定量性能标准可能具有挑战性。本文旨在描述当前可用的Q Orbitrap采集模式的优点和局限性。此外，还讨论了在全扫描实验之间纳入靶向采集。这种方法旨在将需要额外灵敏度或选择性的化合物整合到多残留方法中。
