Department of Microbiology, Faculty of Science and Informatics, University of Szeged, 52. Közép Fasor, 6726 Szeged, Hungary.
Research Group for Food Biotechnology, Institute of Food Science and Technology, Hungarian University of Agriculture and Life Sciences, 1022 Budapest, Hungary.
Toxins (Basel). 2023 Feb 7;15(2):134. doi: 10.3390/toxins15020134.
In routine measurements, the length of the analysis time and nfumber of samples analysed during a time unit are crucial parameters, which are especially important for the food analysis, particularly in the case of mycotoxin determinations. High-resolution equipment, including time-of-flight or Orbitrap analyzators, can provide stable instrumental background for high-throughput analyses. In this report, a short, 1 min MS-based multi-mycotoxin method was developed with the application of a short column as a reduced chromatographic separation, taking advantages of the multiplexing and high-resolution capability of the QExactive Orbitrap MS possessing sub-1 ppm mass accuracy. The performance of the method was evaluated regarding selectivity, LOD, LOQ, linearity, matrix effect, and recovery, and compared to a UHPLC-MS/MS method. The final multiplexing method was able to quantify 11 mycotoxins in defined ranges (aflatoxins (corn, 2.8-600 μg/kg; wheat, 1.5-350 μg/kg), deoxynivalenol (corn, 640-9600 μg/kg; wheat, 128-3500 μg/kg), fumonisins (corn, 20-1500 μg/kg; wheat, 30-3500 μg/kg), HT-2 (corn, 64-5200 μg/kg; wheat, 61-3500 μg/kg), T-2 (corn, 10-800 μg/kg; wheat, 4-250 μg/kg), ochratoxin (corn, 4.7-600 μg/kg; wheat, 1-1000 μg/kg), zearalenone (corn, 64-4800 μg/kg; wheat, 4-500 μg/kg)) within one minute in corn and wheat matrices at the MRL levels stated by the European Union.
在常规测量中，分析时间的长度和单位时间内分析的样品数量是至关重要的参数，这些参数在食品分析中尤为重要，特别是在真菌毒素测定的情况下。高分辨率仪器，包括飞行时间或轨道阱分析仪，可以为高通量分析提供稳定的仪器背景。在本报告中，开发了一种基于质谱的短时间（1 分钟）多真菌毒素方法，应用短柱作为缩短的色谱分离，利用具有亚 1 ppm 质量精度的 QExactive Orbitrap MS 的多路复用和高分辨率能力。该方法的性能通过选择性、LOD、LOQ、线性、基质效应和回收率进行了评估，并与 UHPLC-MS/MS 方法进行了比较。最终的多路复用方法能够在规定范围内定量 11 种真菌毒素（黄曲霉毒素（玉米，2.8-600μg/kg；小麦，1.5-350μg/kg），脱氧雪腐镰刀菌烯醇（玉米，640-9600μg/kg；小麦，128-3500μg/kg），伏马菌素（玉米，20-1500μg/kg；小麦，30-3500μg/kg），HT-2（玉米，64-5200μg/kg；小麦，61-3500μg/kg），T-2（玉米，10-800μg/kg；小麦，4-250μg/kg），赭曲霉毒素（玉米，4.7-600μg/kg；小麦，1-1000μg/kg），玉米赤霉烯酮（玉米，64-4800μg/kg；小麦，4-500μg/kg））在玉米和小麦基质中，在欧盟规定的 MRL 水平下，1 分钟内完成。
