Pei Yifei, Yang Li, Wu Lin, He Huanshan, Geng Guoxia, Xu Dejun, Chen Huali, Li Qingwang
College of Animal Science and Technology, Northwest A&F University, Yangling, Shaanxi Province, China.
College of Veterinary Medicine, Northwest A&F University, Yangling, Shaanxi Province, China.
Anim Sci J. 2018 Jul;89(7):956-965. doi: 10.1111/asj.13009. Epub 2018 Apr 30.
The main aim of the present study was to evaluate the cryoprotective effect of apigenin (AP) and ferulic acid (FA) on boar sperm during cryopreservation. AP and FA were both demonstrated to be high-efficiency antioxidants and had not previously been used to protect sperm from cryodamage. As boar sperm is sensitive to oxidative stress, suitable antioxidants are still needed for improving frozen-thawed sperm quality. With this purpose, semen samples coming from five boars were used in this study. Ejaculates of five boars were mixed and split into 16 aliquots, in which different doses of AP and FA were added separately or together. The motility, the plasma membrane integrity, the mitochondrial activity, the acrosomal integrity, the antioxidase activities and the malondialdehyde concentration of the frozen-thawed boar sperm were assessed. The results suggested that both AP and FA significantly improved the frozen-thawed boar sperm quality in all these aspects when they were added to the freezing extender separately, while the highest improvement was recorded when the extender was supplemented with 0.1 mmol/L AP plus 0.15 mmol/L FA. These findings demonstrated that supplementation of freezing extender with both AP and FA had a combined, beneficial effect on frozen-thawed boar sperm.
本研究的主要目的是评估芹菜素（AP）和阿魏酸（FA）在猪精子冷冻保存过程中的冷冻保护作用。AP和FA均被证明是高效抗氧化剂，且此前未被用于保护精子免受冷冻损伤。由于猪精子对氧化应激敏感，仍需要合适的抗氧化剂来提高冻融后精子的质量。基于此目的，本研究使用了来自五头公猪的精液样本。将五头公猪的射精混合并分成16份，分别或一起添加不同剂量的AP和FA。评估了冻融后猪精子的活力、质膜完整性、线粒体活性、顶体完整性、抗氧化酶活性和丙二醛浓度。结果表明，当AP和FA分别添加到冷冻稀释液中时，在所有这些方面均显著提高了冻融后猪精子的质量，而当稀释液中添加0.1 mmol/L AP加0.15 mmol/L FA时，改善效果最为显著。这些发现表明，在冷冻稀释液中同时添加AP和FA对冻融后猪精子具有联合有益作用。
