Department of Prosthodontics, Ninth People's Hospital, College of Stomatology, Shanghai Jiao Tong University School of Medicine, Shanghai Key Laboratory of Stomatology, Shanghai, China.
Department of Chemistry, Pukyong National University, Busan 48513, South Korea; Marine-Integrated Bionics Research Center, Pukyong National University, Busan 48513, South Korea.
Int J Biol Macromol. 2018 Sep;116:232-238. doi: 10.1016/j.ijbiomac.2018.04.144. Epub 2018 Apr 30.
In our previous study, we showed that Rehmannia glutinosa polysaccharide (RGP) treatment induced maturation of dendritic cells (DCs) and that it had an anticancer effect in mice. The effect of RGP has not been studied in human DCs, including monocyte-derived DCs (MDDCs) and peripheral blood DCs (PBDCs). In this study, we examined DC activation by RGP in human cells. The dendritic morphology of RGP-treated MDDCs was substantially altered as compared with that of phosphate-buffered saline (PBS)-treated control cells. Moreover, RGP treatment markedly decreased phagocytic activity and increased expression levels of co-stimulatory molecules in MDDCs. In addition, RGP treatment elevated the production of proinflammatory cytokines. Furthermore, RGP-induced activation of MDDCs was dependent on the phosphorylation of extracellular signal-regulated kinase (ERK), p38, and c-Jun N-terminal kinase (JNK). RGP-treated MDDCs promoted upregulation of T-cell activation, including proliferation and interferon-gamma (IFN-γ) and tumor necrosis factor-alpha (TNF-α) production. Analysis of the effect of RGP in PBDC subsets revealed that it induced upregulation of co-stimulatory molecule expression and proinflammatory cytokine production. These data suggest that RGP may function as an immune stimulatory molecule in humans.
在我们之前的研究中，我们表明地黄多糖（RGP）处理诱导树突状细胞（DC）成熟，并且在小鼠中具有抗癌作用。RGP 在人类 DC 中的作用尚未得到研究，包括单核细胞衍生的 DC（MDDC）和外周血 DC（PBDC）。在这项研究中，我们研究了 RGP 对人类细胞中 DC 激活的影响。与磷酸盐缓冲盐水（PBS）处理的对照细胞相比，RGP 处理的 MDDC 的树突状形态发生了明显改变。此外，RGP 处理显著降低了 MDDC 的吞噬活性并增加了共刺激分子的表达水平。此外，RGP 处理还增加了促炎细胞因子的产生。此外，RGP 诱导的 MDDC 激活依赖于细胞外信号调节激酶（ERK）、p38 和 c-Jun N-末端激酶（JNK）的磷酸化。RGP 处理的 MDDC 促进 T 细胞激活的上调，包括增殖和干扰素-γ（IFN-γ）和肿瘤坏死因子-α（TNF-α）的产生。对 PBDC 亚群中 RGP 作用的分析表明，它诱导了共刺激分子表达和促炎细胞因子产生的上调。这些数据表明，RGP 可能在人类中作为免疫刺激分子发挥作用。
