Institute of Traditional Chinese Veterinary Medicine, College of Veterinary Medicine, Nanjing Agricultural University, Nanjing 210095, PR China.
Carbohydr Polym. 2013 Jul 25;96(2):516-21. doi: 10.1016/j.carbpol.2013.04.018. Epub 2013 Apr 18.
The aim of this study is to investigate immunomodulatory effect of rehmannia glutinosa polysaccharide (RGP) on murine splenic lymphocyte and bone marrow derived dendritic cells (DCs). Splenic lymphocytes obtained from mice were co-cultured with RGP for 48 h and then harvested for analyzing with MTT method. The cytokine production of T lymphocytes was measured by ELISA. Effects of RGP treatment on DCs were investigated and assessed by MTT method. The results showed RGP significantly stimulated lymphocyte proliferation and the growth rate of T cell was more significant. The IL-2 and IFN-γ production of T lymphocyte were significantly upregulated after being stimulated with RGP. DCs stimulating on proliferation of T cells and the ability of antigen presenting of DCs have been enhanced under the stimulation of RGP. In conclusion, these findings provided valuable information that RGP possessed strong immunoenhancement activity, which provided the theoretical basis for the further experiment.
本研究旨在探讨地黄多糖（RGP）对小鼠脾淋巴细胞和骨髓来源树突状细胞（DC）的免疫调节作用。从小鼠中获得的脾淋巴细胞与 RGP 共培养 48 小时，然后用 MTT 法进行分析。通过 ELISA 法测定 T 淋巴细胞细胞因子的产生。通过 MTT 法研究了 RGP 处理对 DC 的影响。结果表明，RGP 可显著刺激淋巴细胞增殖，T 细胞的增长率更为显著。RGP 刺激后，T 淋巴细胞产生的 IL-2 和 IFN-γ明显上调。在 RGP 的刺激下，DCs 刺激 T 细胞增殖的能力和抗原呈递能力增强。总之，这些发现为 RGP 具有强大的免疫增强活性提供了有价值的信息，为进一步的实验提供了理论依据。
