Jessop J J, Gale K, Bayer B M
Department of Pharmacology, Georgetown University Medical Center, Washington, DC 20007.
Life Sci. 1988;43(14):1133-40. doi: 10.1016/0024-3205(88)90472-9.
The effects of isolation and water scheduling on mitogen induced lymphocyte proliferation were investigated. Isolated rats were animals which had been raised in group-housed conditions and then transferred to individual cages with ad lib access to water for a 1 or 2 week period. Water scheduled rats were maintained in group housing (5 rats per cage) with ad lib access to food but with access to water for a single 30 minute session each day. Responses of these groups were compared to those of animals which had been continuously group-housed with ad lib access to food and water. No differences in lymphocyte responses to phytohemagglutinin (PHA) were found 1 week after exposure to isolation. However, after 2 weeks, splenic and blood T lymphocytes from isolated animals demonstrated an increased proliferative response to suboptimum and maximum concentrations of PHA. Splenic B lymphocyte responses to lipopolysaccharide (LPS) from isolated animals were also increased by 2- to 3-fold compared to group-housed controls. Two weeks of exposure of animals to daily water scheduling similarly increased the splenic lymphocyte proliferation. This increased responsiveness to PHA was not accompanied by a significant change in the sensitivity of the lymphocytes to PHA, in the total number of white blood cells, or the proportion of splenic T or T helper lymphocytes. Our results show that the increase in lymphocyte proliferation is time-dependent, requires greater than 1 week of exposure to isolation and is due to factors other than changes in sensitivity to mitogen or T lymphocyte number.
研究了隔离和水供应计划对丝裂原诱导的淋巴细胞增殖的影响。隔离大鼠是那些先在群居条件下饲养，然后转移到单独笼子里，可自由饮水1或2周的动物。按计划供水的大鼠饲养在群居环境中（每笼5只大鼠），可自由进食，但每天仅能饮水30分钟。将这些组的反应与一直群居且可自由获取食物和水的动物的反应进行比较。暴露于隔离环境1周后，未发现淋巴细胞对植物血凝素（PHA）的反应有差异。然而，2周后，来自隔离动物的脾脏和血液T淋巴细胞对亚最佳和最大浓度的PHA表现出增强的增殖反应。与群居对照相比，来自隔离动物的脾脏B淋巴细胞对脂多糖（LPS）的反应也增加了2至3倍。动物连续2周每天按计划供水同样增加了脾脏淋巴细胞增殖。这种对PHA反应性的增加并未伴随着淋巴细胞对PHA的敏感性、白细胞总数或脾脏T淋巴细胞或T辅助淋巴细胞比例的显著变化。我们的结果表明，淋巴细胞增殖的增加是时间依赖性的，需要暴露于隔离环境超过1周，并且是由对丝裂原敏感性或T淋巴细胞数量变化以外的因素引起的。
