一种鸡肝细胞系能够有效地支持 ALV-J 的复制，可能是通过其高水平的病毒受体和有效的蛋白表达系统。
A chicken liver cell line efficiently supports the replication of ALV-J possibly through its high level viral receptor and efficient protein expression system.
机构信息
Key Laboratory of Jiangsu Preventive Veterinary Medicine, Key Laboratory for Avian Preventive Medicine, Ministry of Education, College of Veterinary Medicine, Yangzhou University, Yangzhou, 225009, Jiangsu, China.
Jiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou, 225009, Jiangsu, China.
出版信息
Vet Res. 2018 May 2;49(1):41. doi: 10.1186/s13567-018-0537-7.
In this study, we identified a chicken liver cell line (LMH) which could strongly support the replication of ALV-J (Subgroup J of avian leukosis virus) with high viral titer. Notably, ALV-J was efficiently detected by ELISA in LMH cells 1 day before DF1 cells. In comparison with DF1 cells, LMH cells not only expressed higher levels of ALV-J receptor chNHE-1, but also possessed a more efficient protein expression system for foreign genes. Thus, LMH cells could be a novel tool to shorten the ALV-J eradication approach and accelerate studies on the pathogenesis and oncogenesis of ALV-J.
在这项研究中，我们鉴定了一个鸡肝细胞系（LMH），它可以强有力地支持 ALV-J（禽白血病病毒的 J 亚群）的复制，具有高病毒滴度。值得注意的是，在 DF1 细胞之前 1 天，LMH 细胞中可以通过 ELISA 高效检测到 ALV-J。与 DF1 细胞相比，LMH 细胞不仅表达更高水平的 ALV-J 受体 chNHE-1，而且还具有更有效的外源基因蛋白表达系统。因此，LMH 细胞可能是一种缩短 ALV-J 根除方法和加速研究 ALV-J 发病机制和致癌机制的新工具。
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