Cheng Chunhong, Li Changman, Wang Diandong, Zhai Lifeng, Cai Zhaoming
College of Life Science and Technology, Yangtze Normal University, Chongqing, China.
Front Plant Sci. 2018 Apr 18;9:514. doi: 10.3389/fpls.2018.00514. eCollection 2018.
( nodule autoregulation receptor kinase) is the homolog of () and one of the most important regulators in the process of AON (Autoregulation of Nodulation), a process that restricts excessive nodule numbers in soybean. However, except for the function in AON, little is known about this gene. Here, we report that GmNARK plays important roles in process of plant response to abiotic stresses. Bioinformatic analysis and subcellular localization experiment results showed that GmNARK was a putative receptor like kinase and located at membrane. The promoter of contains manifold regulatory elements that are responsive to hormone and stresses. Gene transcript expression pattern analysis in soybean revealed was induced by ABA and NaCl treatment in both shoot and root. Overexpression of GmNARK in resulted in higher sensitivity to ABA and salt treatment during seed germination and greening stages. We also checked the expression levels of some ABA response genes in the transgenic lines; the results showed that the transcript level of all the ABA response genes were much higher than that of wild type under ABA treatment. Our results revealed a novel role of GmNARK in response to abiotic stresses during plant growth and development.
（结节自调控受体激酶）是（）的同源物，也是大豆中限制根瘤数量过多的结瘤自调控（AON）过程中最重要的调控因子之一。然而，除了在AON中的功能外，对该基因知之甚少。在此，我们报道GmNARK在植物对非生物胁迫的响应过程中发挥重要作用。生物信息学分析和亚细胞定位实验结果表明，GmNARK是一种假定的类受体激酶，位于细胞膜上。（此处原文缺失相关基因名称，无法准确翻译）的启动子包含多种对激素和胁迫有响应的调控元件。大豆中的基因转录表达模式分析表明，在地上部和根部，（此处原文缺失相关基因名称，无法准确翻译）均受脱落酸（ABA）和氯化钠（NaCl）处理诱导。在（此处原文缺失相关受体名称，无法准确翻译）中过表达GmNARK导致种子萌发和绿化阶段对ABA和盐处理的敏感性更高。我们还检测了转基因株系中一些ABA响应基因的表达水平；结果表明，在ABA处理下，所有ABA响应基因的转录水平均远高于野生型。我们的结果揭示了GmNARK在植物生长发育过程中对非生物胁迫响应中的新作用。
