School of Chemistry and Chemical Engineering, Jiangsu University, Zhenjiang, Jiangsu 212013, P. R. China.
Analyst. 2018 May 29;143(11):2555-2562. doi: 10.1039/c8an00556g.
Labile Fe2+ has been considered to be a metabolically active and regulatory form of cellular iron. Monitoring the dynamic level of labile Fe2+ in biological systems is vital for evaluating the iron related biological processes and diseases as well as dissecting the exact physiological and pathophysiological functions of the labile Fe2+. Herein, we rationally constructed a coumarin-based fluorescent probe for sensing labile Fe2+ in living systems based on a novel Fe2+ meditated cyclization reaction strategy. The probe showed a highly selective and sensitive response to Fe2+, and the detection limit was determined to be 45 nM. Significantly, the probe displayed fast response to Fe2+, with the sensing reaction completed in 2 min, which is beneficial for real time sensing. The application of the probe for sensing different concentrations of labile Fe2+ in living cells has been conducted. In addition, the basal and endogenous levels of labile Fe2+ in living systems were also successfully monitored.
不稳定的 Fe2+ 被认为是细胞铁的一种代谢活跃和调节形式。监测生物系统中不稳定的 Fe2+ 的动态水平对于评估与铁相关的生物过程和疾病以及剖析不稳定的 Fe2+ 的精确生理和病理生理功能至关重要。在此，我们基于一种新的 Fe2+介导的环化反应策略，合理构建了一种基于香豆素的荧光探针，用于在活细胞中检测不稳定的 Fe2+。该探针对 Fe2+表现出高度选择性和灵敏的响应，检测限为 45 nM。值得注意的是，探针对 Fe2+的响应非常迅速，在 2 分钟内完成传感反应，这有利于实时传感。该探针已用于检测活细胞中不同浓度的不稳定 Fe2+。此外，还成功监测了活系统中不稳定的 Fe2+的基础和内源性水平。
