Co-Innovation Center of Efficient Processing and Utilization of Forest Resources, College of Chemical Engineering, College of Light Industry and Food, Nanjing Forestry University, Nanjing, 210037, China.
Anal Bioanal Chem. 2021 Oct;413(25):6267-6277. doi: 10.1007/s00216-021-03581-4. Epub 2021 Aug 6.
In this work, a novel fluorescent probe CBO was synthesized for detecting Fe using the natural monoterpenketone camphor as the starting material. The probe CBO displayed turn-on fluorescence to Fe accompanied by the solution change from colorless to green. As expected, there was an excellent linear relationship between the fluorescence intensity of probe CBO and the concentration of Fe (0-20 μM), and the detection limit was as low as 1.56×10 M. In particular, CBO could selectively sense Fe more than other analytes (Fe included) through the N-oxide strategy, and quickly responded to Fe (60 s) over a wide pH (4-14) range. Additionally, based on the rapid fluorescence response of CBO to Fe, a simple test strip-based detector was designed for boosting practical applicability. The probe CBO had been successfully applied to the fluorescence imaging of Fe in onion cells and living zebrafish. The probe CBO was a powerful tool of detecting Fe level in organisms, which was of significance to understand the role of Fe in Fe-related physical processes and diseases.
在这项工作中，我们合成了一种新型荧光探针 CBO，该探针以天然单萜酮樟脑为起始原料，用于检测 Fe。探针 CBO 对 Fe 的检测表现出开启型荧光，同时溶液颜色由无色变为绿色。不出所料，探针 CBO 的荧光强度与 Fe 的浓度（0-20 μM）之间存在极好的线性关系，检测限低至 1.56×10 M。特别地，CBO 可以通过 N-氧化物策略选择性地检测 Fe 及其它分析物（包括 Fe），并在较宽的 pH 值（4-14）范围内快速响应 Fe（60 s）。此外，基于 CBO 对 Fe 的快速荧光响应，我们设计了一种简单的基于试条的探测器，以提高实际适用性。探针 CBO 已成功应用于洋葱细胞和活体斑马鱼中 Fe 的荧光成像。CBO 是一种检测生物体内 Fe 水平的有力工具，这对于理解 Fe 在与 Fe 相关的物理过程和疾病中的作用具有重要意义。
