Department of Ophthalmology, USC Eye Institute, Keck School of Medicine of the University of Southern California, Los Angeles, California, USA.
USC Roski Eye Institute, Los Angeles, CA, USA.
Adv Exp Med Biol. 2018;1074:157-166. doi: 10.1007/978-3-319-75402-4_20.
Purpose This study demonstrates a standardized approach to measuring retinal thickness (RT) using spectral domain optical coherence tomography (SD-OCT) in commonly used animal models of disease and reports a normative data set for future use. Materials and Methods Twenty normal eyes of 4 adult animal models (5 rats, 5 rabbits, 5 canines, and 5 mini-pigs) were used. Manual measurements were made on the commercially available Heidelberg Spectralis™ SD-OCT to determine the total, inner, and outer retinal thickness (RT) at fixed distances from the optic nerve head (ONH) (1, 2, 3, 4, 5, and 6 mm away) in order to control for normal variation in retinal thickness. Analysis of variance (ANOVA) with P value <0.05 indicated statistical significance. Results Total RT significantly decreased with increasing distance from the ONH for the canine, mini-pig, and rabbit vascular models. Inner RT significantly decreased for the canine, mini-pig, rabbit vascular, and rabbit avascular models; and outer RT significantly decreased for only the canine model. Among the animal models, RT at similar distances from the ONH were significantly different for total, inner, and outer RT. Conclusion There are significant differences in the total, inner, and outer RT of normal canine, mini-pig, rabbit, and rat retinas with SD-OCT using a standardized approach. These measurements provide a normative reference for future studies and illustrate a standardized method of assessing RT.
目的 本研究采用谱域光学相干断层扫描（SD-OCT），对疾病常用的动物模型进行视网膜厚度（RT）的标准化测量，并提供未来使用的正常参考数据集。
材料和方法 使用 4 种成年动物模型（5 只大鼠、5 只兔子、5 只犬和 5 只小型猪）的 20 只正常眼。在商用海德堡 Spectralis™ SD-OCT 上进行手动测量，以确定距视神经头（ONH）不同固定距离（距 ONH 1、2、3、4、5 和 6mm 处）的全层、内和外层 RT，以控制视网膜厚度的正常变化。P 值<0.05 的方差分析（ANOVA）表示具有统计学意义。
结果 犬、小型猪和兔血管模型的全层 RT 随距 ONH 距离的增加而显著降低。犬、小型猪、兔血管和兔非血管模型的内层 RT 显著降低；仅犬模型的外层 RT 显著降低。在这些动物模型中，距 ONH 相似距离的全层、内和外层 RT 存在显著差异。
结论 使用标准化方法的 SD-OCT 测量正常犬、小型猪、兔和大鼠视网膜的全层、内和外层 RT 存在显著差异。这些测量值为未来的研究提供了正常参考，并说明了一种评估 RT 的标准化方法。
