Institute of Molecular and Clinical Ophthalmology Basel (IOB), 4031, Basel, Switzerland.
Department of Ophthalmology, University Hospital Basel, 4031, Basel, Switzerland.
Sci Rep. 2022 Aug 2;12(1):13276. doi: 10.1038/s41598-022-17699-7.
Cynomolgus monkeys exhibit human-like features, such as a fovea, so they are often used in non-clinical research. Nevertheless, little is known about the natural variation of the choroidal thickness in relation to origin and sex. A combination of deep learning and a deterministic computer vision algorithm was applied for automatic segmentation of foveolar optical coherence tomography images in cynomolgus monkeys. The main evaluation parameters were choroidal thickness and surface area directed from the deepest point on OCT images within the fovea, marked as the nulla with regard to sex and origin. Reference choroid landmarks were set underneath the nulla and at 500 µm intervals laterally up to a distance of 2000 µm nasally and temporally, complemented by a sub-analysis of the central bouquet of cones. 203 animals contributed 374 eyes for a reference choroid database. The overall average central choroidal thickness was 193 µm with a coefficient of variation of 7.8%, and the overall mean surface area of the central bouquet temporally was 19,335 µm and nasally was 19,283 µm. The choroidal thickness of the fovea appears relatively homogeneous between the sexes and the studied origins. However, considerable natural variation has been observed, which needs to be appreciated.
食蟹猴具有人眼的特征，例如中央凹，因此常被用于非临床研究。然而，关于脉络膜厚度与起源和性别的自然变异知之甚少。本研究应用深度学习和确定性计算机视觉算法，对食蟹猴的中心凹光学相干断层扫描图像进行自动分割。主要评估参数是来自 OCT 图像中心凹最深处的脉络膜厚度和面积，标记为关于性别和起源的“无”。参考脉络膜标志点设置在“无”下方，以及在鼻侧和颞侧以 500µm 的间隔向外侧延伸 2000µm，并对中央圆锥体束进行了补充分析。203 只动物为参考脉络膜数据库贡献了 374 只眼睛。中央脉络膜的平均总厚度为 193µm，变异系数为 7.8%，中央圆锥体束的总表面积在颞侧为 19335µm，鼻侧为 19283µm。在性别和研究起源之间，中央凹的脉络膜厚度似乎相对均匀。然而，已经观察到相当大的自然变异，需要加以重视。
