University Hospital Essen, Department of Neurology, Essen, Germany.
Department für Augenheilkunde, Forschungsinstitut für Augenheilkunde, Eberhard Karls Universität Tübingen, Tübingen, Germany.
Adv Exp Med Biol. 2018;1074:511-517. doi: 10.1007/978-3-319-75402-4_63.
In humans cone photoreceptors are responsible for high-resolution colour vision. A variety of retinal diseases can compromise cone viability, and, at present, no satisfactory treatment options are available. Here, we present data towards establishing a reliable, high-throughput assay system that will facilitate the search for cone neuroprotective compounds using the murine-photoreceptor cell line 661 W. To further characterize 661 W cells, a retinal marker study was performed, followed by the induction of cell death using paradigms over-activating cGMP-dependent protein kinase G (PKG). We found that 661 W cells may be used to mimic specific aspects of cone degeneration and may thus be valuable for future compound screening studies.
在人类中，视锥细胞负责高分辨率的色彩视觉。多种视网膜疾病会损害视锥细胞的活力，而目前尚无可令人满意的治疗方法。在这里，我们提出了建立可靠的高通量检测系统的数据，这将有助于使用小鼠感光细胞系 661W 寻找视锥细胞神经保护化合物。为了进一步描述 661W 细胞，我们进行了视网膜标志物研究，然后使用过度激活 cGMP 依赖性蛋白激酶 G（PKG）的范例诱导细胞死亡。我们发现，661W 细胞可用于模拟视锥细胞退化的特定方面，因此对未来的化合物筛选研究可能具有重要价值。
